CAS Number369-07-3
SynonymsEINECS 206-716-1; T6OTJ BOR BNW& CQ DQ EQ F1Q &&β-D-Galacto Form; MFCD00063255; 2-Nitrophenyl β-D-galactoside; ortho-Nitrophenyl-β-galactoside; ONPG
Molecular FormulaC12H15NO8
Molecular Weight301.25
Purity≥98 (enzymatic)%
Density1.6±0.1 g/cm3
Boiling Point572.4±50.0 °C at 760 mmHg
Melting Point186-193 °C (dec.)
Appearancepowder
EINECS206-716-1
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| Chemical & Physical Properties | |
|---|---|
| CAS Number | 369-07-3 |
| Catalog No. | 2A-9021555 |
| Chinese Name | 邻硝基苯-β-D-半乳糖苷 |
| Synonyms | EINECS 206-716-1; T6OTJ BOR BNW& CQ DQ EQ F1Q &&β-D-Galacto Form; MFCD00063255; 2-Nitrophenyl β-D-galactoside; ortho-Nitrophenyl-β-galactoside; ONPG |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 |
| Purity | ≥98 (enzymatic) |
| Density | 1.6±0.1 g/cm3 |
| Boiling Point | 572.4±50.0 °C at 760 mmHg |
| Melting Point | 186-193 °C (dec.) |
| Appearance | powder |
| Storage Condition | Store at 2-8℃. |
| Application | ONPG is a colorimetric and spectrophotometric substrate for the detection of beta-galactosidase activity. |
| EINECS | 206-716-1 |
| HTC | 29400090 |
| PubChem ID | 24897461 |
| MDL Number | MFCD00063255 |
| SMILES | OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O |
| InChI | 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 |
| InChI Key | KUWPCJHYPSUOFW-YBXAARCKSA-N |
| Beilstein/REAXYS | 92207 |
| NACRES Code | NA.52 |
| UNSPSC | 12352200 |
| Hazard Symbols | Transport |
| MSDS | msds/21555_1_369_07_3.pdf |
| Bioactivity | ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. Related Catalog Signaling Pathways >> Others >> Others Biochemical Assay Reagents Research Areas >> Others In Vitro The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1]. Kinase Assay The β-galactosidase activity is measured using two substrates including ONPG and lactose in this study. The β-galactosidase activity for ONPG is measured by following the amount o-nitrophenol released from ONPG. The reaction mixture is composed of 100 μL of the enzyme solution and 400 μL of ONPG solution (2.5 g/L in 100 mM Tris-HCl buffer at pH 6.8). After incubation at 78°C for 15 min, the reaction is terminated by adding an equal volume of 1 M Na2CO3. The released o-nitrophenol is quantitatively determined by measuring at A405. One unit of activity is defined as the amount of enzyme needed to produce 1 μmol of o-nitrophenol per minute under the assay condition. The specific activity is expressed as units per milligram of protein. Assays for activity towards lactose are performed in the same buffer containing 100 μL of enzyme solution and 5% lactose, and the reaction is stopped by boiling for 10 min, and the concentration of glucose is determined using a glucose oxidase-peroxidase assay kit. The released glucose is quantitatively determined by measuring A492. One unit of enzyme activity is defined as the amount of activity required to release 1 μmol of glucose per minute[1]. References [1]. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237. doi: 10.1186/1471-2180-13-237. Chemical & Physical Properties Density 1.6±0.1 g/cm3 Boiling Point 572.4±50.0 °C at 760 mmHg Melting Point 186-193 °C (dec.) Molecular Formula C12H15NO8 Molecular Weight 301.249 Flash Point 300.0±30.1 °C Exact Mass 301.079773 PSA 145.20000 LogP -0.78 Vapour Pressure 0.0±1.7 mmHg at 25°C Index of Refraction 1.648 InChIKey KUWPCJHYPSUOFW-YBXAARCKSA-N SMILES O=[N+]([O-])c1ccccc1OC1OC(CO)C(O)C(O)C1O Storage condition 2~8°C |
| Use of | ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. Properties Articles81 Name 2-Nitrophenyl-beta-D-galactopyranoside Synonym More Synonyms ONPG Biological Activity Description ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. Related Catalog Signaling Pathways >> Others >> Others Biochemical Assay Reagents Research Areas >> Others References Chemical & Physical Properties Molecular Formula C12H15NO8 Exact Mass 301.079773 PSA 145.20000 LogP -0.78 Index of Refraction 1.648 InChIKey KUWPCJHYPSUOFW-YBXAARCKSA-N |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available See reverse side of invoice or packing slip. |
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