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ABTS structure, CAS 30931-67-0

ABTS

CAS Number30931-67-0

Synonyms2,2'-Azinobis(3-ethylbenzothiazolin-6-sulfonsaeure)diammoniumsalz; AzBTS-(NH4)2; 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; ABTS (diammonium salt); ABTS SUBSTRATE; diammonium 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate); ABTS ammonium salt; ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)]; MFCD00010404; 2,2'-azinobis-(3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate) diammonium salt; 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt); ABTS-(NH4)2; 2,2'-(1,4-PHENYLENE)BIS-1H-BENZOTHIAZOLE; EINECS 250-396-6; ABTS(R),DIAMMONIUM SALT; ABTS substrate tablets; ABTS

Molecular FormulaC18H24N6O6S4

Molecular Weight548.68

Purity≥98 (HPLC)%

Density

Boiling Point881.2ºC at 760mmHg

Melting Point

Flash Point

Appearancepowder

EINECS250-396-6

MSDSChineseEnglish

Symbol

Signal Word

Cat. No.: 2A-9009026 Purity: ≥98 (HPLC)%
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Quality Control of [ 30931-67-0 ] Purity: ≥98 (HPLC)% SDS Specification MoL

COA NMR CNMR HPLC LC-MS
Chemical & Physical Properties
CAS Number30931-67-0
Catalog No.2A-9009026
Chinese Name2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐
Synonyms2,2'-Azinobis(3-ethylbenzothiazolin-6-sulfonsaeure)diammoniumsalz; AzBTS-(NH4)2; 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; ABTS (diammonium salt); ABTS SUBSTRATE; diammonium 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate); ABTS ammonium salt; ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)]; MFCD00010404; 2,2'-azinobis-(3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate) diammonium salt; 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt); ABTS-(NH4)2; 2,2'-(1,4-PHENYLENE)BIS-1H-BENZOTHIAZOLE; EINECS 250-396-6; ABTS(R),DIAMMONIUM SALT; ABTS substrate tablets; ABTS
Molecular FormulaC18H24N6O6S4
Molecular Weight548.68
Purity≥98 (HPLC)
Boiling Point881.2ºC at 760mmHg
Appearancepowder
Water SolubilityH2O: 50 mg/mL, very slightly hazy, green
Storage ConditionSealed, stored in greenhouse
ApplicationABTS diammonium salt is a substrate for horseradish peroxidase (HRP).
EINECS250-396-6
HTC29342080
PubChem ID24890599
MDL NumberMFCD00010404
SMILES[NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
InChI1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI KeyOHDRQQURAXLVGJ-AXMZSLBLSA-N
Beilstein/REAXYS7329461
NACRES CodeNA.83
UNSPSC12352204
MSDSmsds/9026_1_30931_67_0.pdf
BioactivityABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate. Related Catalog Signaling Pathways >> Others >> Others Biochemical Assay Reagents Research Areas >> Others In Vitro A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H2O2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H2O2. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM[2]. Kinase Assay Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H2O2 concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H2O2 concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε420 36,000 L mol-1 cm-1)[2]. References [1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164. Chemical & Physical Properties Boiling Point 881.2ºC at 760mmHg Molecular Formula C18H24N6O6S4 Molecular Weight 548.680 Flash Point 486.7ºC Exact Mass 548.064026 PSA 223.04000 LogP 5.47820 InChIKey OHDRQQURAXLVGJ-UHFFFAOYSA-N SMILES CCn1c(=NN=c2sc3cc(S(=O)(=O)[O-])ccc3n2CC)sc2cc(S(=O)(=O)[O-])ccc21.[NH4+].[NH4+] Water Solubility H2O: 50 mg/mL, very slightly hazy, green
Use ofABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate. Properties Articles45 Name Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) Synonym More Synonyms ABTS Biological Activity Description ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate. Related Catalog Signaling Pathways >> Others >> Others Biochemical Assay Reagents Research Areas >> Others References [1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164. Chemical & Physical Properties Molecular Formula C18H24N6O6S4 Exact Mass 548.064026 PSA 223.04000 LogP 5.47820 InChIKey OHDRQQURAXLVGJ-UHFFFAOYSA-N
Transport InfoProduct name: ABTS [=2,2'-azobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt]
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