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Acridine Orange 10-nonyl bromide structure, CAS 75168-11-5

Acridine Orange 10-nonyl bromide

CAS Number75168-11-5

Synonyms3-N,3-N,6-N,6-N-tetramethyl-10-nonylacridin-10-ium-3,6-diamine,bromide; MFCD00467124

Molecular FormulaC26H38BrN3

Molecular Weight472.50

Purity≥90 (HPLC)%

Density

Boiling Point

Melting Point≥250 °C (lit.)

Flash Point

Appearancepowder or solid

EINECS

MSDSChineseEnglish

Symboltransportation

Signal WordWarning

Cat. No.: 2A-9047835 Purity: ≥90 (HPLC)%
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Quality Control of [ 75168-11-5 ] Purity: ≥90 (HPLC)% SDS Specification MoL

COA NMR CNMR HPLC LC-MS
Chemical & Physical Properties
CAS Number75168-11-5
Catalog No.2A-9047835
Chinese Name10-壬基吖啶橙溴化物
Synonyms3-N,3-N,6-N,6-N-tetramethyl-10-nonylacridin-10-ium-3,6-diamine,bromide; MFCD00467124
Molecular FormulaC26H38BrN3
Molecular Weight472.50
Purity≥90 (HPLC)
Melting Point≥250 °C (lit.)
Appearancepowder or solid
Storage Condition2~8°C
ApplicationAcridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).
HTC2933990090
PubChem ID329770942
MDL NumberMFCD00467605
SMILES[Br-].CCCCCCCCC[n+]1c2cc(ccc2cc3ccc(cc13)N(C)C)N(C)C
InChI1S/C26H38N3.BrH/c1-6-7-8-9-10-11-12-17-29-25-19-23(27(2)3)15-13-21(25)18-22-14-16-24(28(4)5)20-26(22)29;/h13-16,18-20H,6-12,17H2,1-5H3;1H/q+1;/p-1
InChI KeyDRBHTUDHPPBMCD-UHFFFAOYSA-M
NACRES CodeNA.47
UNSPSC12171500
Hazard Symbolstransportation
BioactivityAcridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm). Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vivo Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2]. Cell Assay Yeast cells in log phase are fixed in cold ethanol (70% by vol.) and stored at -20°C. Fixed cells are washed three times with cold 10 mM Tris/HCI pH 7, then mildly sonicated to eliminate aggregates and finally counted. Yeast cells are added to 45 μM Acridine Orange 10-Nonyl Bromide and incubated for 15 min at 20°C. Cells are centrifuged (3000×g, 5 min) then washed twice in 10 mM Tris/HCl pH 7. Red fluorescence of Acridine Orange 10-Nonyl Bromide bound to 106 yeast cells is measured at 640 nm and correlated to the calibration curve run with thin-walled vesicles containing known amounts of cardiolipin[2]. References [1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12. [2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9. Chemical & Physical Properties Melting Point ≥250ºC(lit.) Molecular Formula C26H38BrN3 Molecular Weight 472.50400 Exact Mass 471.22500 PSA 10.36000 LogP 3.16710 InChIKey DRBHTUDHPPBMCD-UHFFFAOYSA-M SMILES CCCCCCCCC[n+]1c2cc(N(C)C)ccc2cc2ccc(N(C)C)cc21.[Br-] Storage condition 2~8°C
Use ofAcridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm). Properties Name Acridine Orange 10-nonyl bromide Synonym More Synonyms Acridine Orange 10-Nonyl Bromide Biological Activity Description Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm). Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vivo Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2]. References [1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12. [2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9. Chemical & Physical Properties Molecular Formula C26H38BrN3 Exact Mass 471.22500 PSA 10.36000 LogP 3.16710 InChIKey DRBHTUDHPPBMCD-UHFFFAOYSA-M
Tax Rebate13.0%
SupervisionNone. MFN tariff: 6.5%. Ordinary tariff: 20.0%
Transport InfoShipping Name: UN
MSDS Transport InfoModule 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available The above information is believed to be correct, but is not all-inclusive and should be used as a guide only. The information in this document is based on our current knowledge and is Correct safety instructions apply to this product. This information does not represent a warranty as to the properties of this product. See reverse side of invoice or packing slip.
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