
CAS Number41830-80-2
SynonymsCRESYL VIOLET PERCHLORATE; Cresyl Violet 670; CRESYL VIOLET 670 PERCHLORATE); EINECS 255-561-6; Oxazine 9 perchlorate; Cresyl Violet perchlorate,laser grade,pure; Cresyl Violet perchlorate,laser grade; Cresylvioletperchlorate,lasergrade; MFCD00012671; Cresyl Violet perchlorate,pure,laser grade
Molecular FormulaC16H12ClN3O5
Molecular Weight361.73700
Purity98.00%
Boiling Point482.3ºC at 760mmHg
Melting Point331 - 333ºC
Appearancedark green crystal
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 41830-80-2 |
| Catalog No. | 2A-9042867 |
| Chinese Name | 甲酚紫高氯酸盐 |
| Synonyms | CRESYL VIOLET PERCHLORATE; Cresyl Violet 670; CRESYL VIOLET 670 PERCHLORATE); EINECS 255-561-6; Oxazine 9 perchlorate; Cresyl Violet perchlorate,laser grade,pure; Cresyl Violet perchlorate,laser grade; Cresylvioletperchlorate,lasergrade; MFCD00012671; Cresyl Violet perchlorate,pure,laser grade |
| Molecular Formula | C16H12ClN3O5 |
| Molecular Weight | 361.73700 |
| Purity | 98.00 |
| Boiling Point | 482.3ºC at 760mmHg |
| Melting Point | 331 - 333ºC |
| Appearance | dark green crystal |
| Storage Condition | Sealed in a cool, dry environment |
| Application | Cresyl violet perchlorate is a red fluorescent stain that can be used to stain neurons. |
| PubChem ID | 3156597 |
| SMILES | Nc1ccc2nc3c4ccccc4c(=[NH2+])cc-3oc2c1.[O-][Cl+3]([O-])([O-])[O-] |
| InChI | InChI=1S/C19H18N3O.ClH/c1-11-8-15-17(10-16(11)22(2)3)23-18-9-14(20)12-6-4-5-7-13(12)19(18)21-15;/h4-10H,20H2,1-3H3;1H/q+1;/p-1 |
| InChI Key | HLHUTAPXZITNPJ-UHFFFAOYSA-N |
| Hazard Symbols | transportation |
| Bioactivity | Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Neurological Disease In Vitro The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1]. Cell Assay Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1]. References [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6. Chemical & Physical Properties Boiling Point 482.3ºC at 760mmHg Melting Point 331 - 333ºC Molecular Formula C16H12ClN3O5 Molecular Weight 361.73700 Flash Point 245.5ºC Exact Mass 361.04700 PSA 147.34000 LogP 3.98470 Index of Refraction 1.758 InChIKey HLHUTAPXZITNPJ-UHFFFAOYSA-N SMILES Nc1ccc2nc3c4ccccc4c(=[NH2+])cc-3oc2c1.[O-][Cl+3]([O-])([O-])[O-] |
| Use of | Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons. Properties Articles25 Name cresyl violet perchlorate Synonym More Synonyms Cresyl Violet perchlorate Biological Activity Description Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Neurological Disease References [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6. Chemical & Physical Properties Exact Mass 361.04700 PSA 147.34000 LogP 3.98470 Index of Refraction 1.758 InChIKey HLHUTAPXZITNPJ-UHFFFAOYSA-N |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 Names specified by the United Nations (UN) European Land Transport Dangerous Regulations: No dangerous goods IMDG Code: No dangerous goods International Air Transport Dangerous Regulations: No dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Dangerous Regulations: No International Maritime Transport Dangerous Regulations Maritime Pollutants: No International Air Transport Risk Regulations: No 14.6 Special reminder to users No data available |
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