CAS Number1239-45-8
Synonyms3,8-Diamino-5; MFCD00011724; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide; Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1); Homidium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; EINECS 214-984-6; 5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide; EthidiuM BroMide
Molecular FormulaC21H20BrN3
Molecular Weight394.31
Purity98.00%
Melting Point260-262 °C (dec.)(lit.)
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 1239-45-8 |
| Catalog No. | 2A-9026538 |
| Chinese Name | 溴化乙啶 |
| Synonyms | 3,8-Diamino-5; MFCD00011724; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide; Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1); Homidium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; EINECS 214-984-6; 5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide; EthidiuM BroMide |
| Molecular Formula | C21H20BrN3 |
| Molecular Weight | 394.31 |
| Purity | 98.00 |
| Melting Point | 260-262 °C (dec.)(lit.) |
| Appearance | powder |
| Water Solubility | 40 g/L (25 ºC) |
| Storage Condition | Sealed, store at 2 ºC -8 ºC |
| Packaging | I |
| Application | Ethidium bromide is often used as a fluorescent label (nucleic acid stain) in molecular biology experiments, such as agarose gel electrophoresis. |
| HTC | 29339990 |
| UN(IATA) | 2811 |
| PubChem ID | 57650684 |
| SMILES | CC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc21.[Br-] |
| InChI | InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H |
| InChI Key | ZMMJGEGLRURXTF-UHFFFAOYSA-N |
| Hazard Symbols | 6.1 |
| Bioactivity | Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The preparation of agarose gel: 1． Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated. 2． Mix the agarose powder with 1X TAE/TBE. 3． Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil). 4． Make the solution cool down before solidification. 5． Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 µL stock/100 mL gel). 6． Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light. CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection. References [1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0. Chemical & Physical Properties Melting Point 260-262 °C (dec.)(lit.) Molecular Formula C21H20BrN3 Molecular Weight 394.308 Flash Point >100°C Exact Mass 393.084045 PSA 55.92000 LogP 2.29820 Index of Refraction 1.4575 (20ºC) InChIKey ZMMJGEGLRURXTF-UHFFFAOYSA-N SMILES CC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc21.[Br-] Storage condition 2-8°C Stability Stable. Incompatible with strong oxidizing agents. Water Solubility 40 g/L (25 ºC) |
| Use of | Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. Properties Articles1080 Name ethidium bromide Synonym More Synonyms Ethidium bromide Biological Activity Description Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others References [1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0. Chemical & Physical Properties Molecular Formula C21H20BrN3 Exact Mass 393.084045 PSA 55.92000 LogP 2.29820 Index of Refraction 1.4575 (20ºC) InChIKey ZMMJGEGLRURXTF-UHFFFAOYSA-N Stability Stable. Incompatible with strong oxidizing agents. |
| Tax Rebate | 13.0% |
| Supervision | None. MFN tariff: 6.5%. Ordinary tariff: 20.0% |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Danger Regulations: 2810 International Maritime Transport Danger Regulations: 2810 International Air Transport Danger Regulations: 2810 14.2 United Nations shipping name European Land Transport Dangerous Regulations: TOXIC LIQUID, ORGANIC, N.O.S. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide) IMDG Code: TOXIC LIQUID, ORGANIC, N.O.S. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide) International air transport hazard regulations: Toxic liquid, organic, n.o.s. (3,8-Diamino-1-ethyl-6-phenylphenantridinium bromide) 14.3 Transport hazard categories European Land Transport Danger Regulations: 6.1 International Maritime Transport Danger Regulations: 6.1 International Air Transport Danger Regulations: 6.1 14.4 Package group European Land Transport Danger Regulations: III International Maritime Transport Danger Regulations: III International Air Transport Danger Regulations: III 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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