CAS Number76-59-5
SynonymsPhenol, 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1-methylethyl)-, S,S-dioxide; EINECS 200-971-2; a,a-Bis(6-bromo-5-hydroxycarvacryl)-a-hydroxy-o-toluenesulfonic Acid g-Sultone; 4,4'-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis[2-bromo-3-methyl-6-(propan-2-yl)phenol]; 4,4'-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1-methylethyl)phenol] S,S-Dioxide; 3',3''-Dibromothymolsulfonphthalein; Bromthymol Blue; 4,4'-(1,1-Dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2-bromo-6-isopropyl-3-methylphenol); Bromothymol blue; MFCD00005872
Molecular FormulaC27H28Br2O5S
Molecular Weight624.38
Purity98.00%
Density1.5±0.1 g/cm3
Boiling Point614.3±55.0 °C at 760 mmHg
Melting Point200-202 °C ((392 - 396 °F) - lit.)
Appearancepowder
EINECS200-971-2
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 76-59-5 |
| Catalog No. | 2A-9017145 |
| Chinese Name | 溴百里香酚蓝(BTB) |
| Synonyms | Phenol, 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1-methylethyl)-, S,S-dioxide; EINECS 200-971-2; a,a-Bis(6-bromo-5-hydroxycarvacryl)-a-hydroxy-o-toluenesulfonic Acid g-Sultone; 4,4'-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis[2-bromo-3-methyl-6-(propan-2-yl)phenol]; 4,4'-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1-methylethyl)phenol] S,S-Dioxide; 3',3''-Dibromothymolsulfonphthalein; Bromthymol Blue; 4,4'-(1,1-Dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2-bromo-6-isopropyl-3-methylphenol); Bromothymol blue; MFCD00005872 |
| Molecular Formula | C27H28Br2O5S |
| Molecular Weight | 624.38 |
| Purity | 98.00 |
| Density | 1.5±0.1 g/cm3 |
| Boiling Point | 614.3±55.0 °C at 760 mmHg |
| Melting Point | 200-202 °C ((392 - 396 °F) - lit.) |
| Appearance | powder |
| Water Solubility | slightly soluble |
| Storage Condition | This product should be sealed and stored in a cool |
| Packaging | III |
| Application | Bromothymol Blue is a pH indicator that is used when measuring weak acids and weak bases. It is yellow when it is acidic and blue when it is alkaline. |
| EINECS | 200-971-2 |
| HTC | 29349990 |
| PubChem ID | 24847171 |
| MDL Number | MFCD00005872 |
| SMILES | CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C |
| InChI | 1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3 |
| InChI Key | NUHCTOLBWMJMLX-UHFFFAOYSA-N |
| Beilstein/REAXYS | 373934 |
| NACRES Code | NA.47 |
| UNSPSC | 12171500 |
| Hazard Symbols | Transport |
| MSDS | msds/17145_1_76_59_5.pdf |
| Bioactivity | Bromothymol Blue is a pH indicator. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2]. Kinase Assay For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress(DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL BTB and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1]. References [1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80. [2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul - Sep;48(3):397-402. Chemical & Physical Properties Density 1.5±0.1 g/cm3 Boiling Point 614.3±55.0 °C at 760 mmHg Melting Point 204 °C Molecular Formula C27H28Br2O5S Molecular Weight 624.381 Flash Point 325.3±31.5 °C Exact Mass 622.002380 PSA 92.21000 LogP 8.60 Vapour Pressure 0.0±1.8 mmHg at 25°C Index of Refraction 1.634 InChIKey NUHCTOLBWMJMLX-UHFFFAOYSA-N SMILES Cc1c(C2(c3cc(C(C)C)c(O)c(Br)c3C)OS(=O)(=O)c3ccccc32)cc(C(C)C)c(O)c1Br Storage condition Store at RT. Stability Stable. Incompatible with strong oxidizing agents. Water Solubility slightly soluble |
| Use of | Bromothymol Blue is a pH indicator. Properties Articles33 Name bromothymol blue Synonym More Synonyms Bromothymol blue Biological Activity Description Bromothymol Blue is a pH indicator. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others References [1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80. [2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul - Sep;48(3):397-402. Chemical & Physical Properties Molecular Formula C27H28Br2O5S Exact Mass 622.002380 PSA 92.21000 Index of Refraction 1.634 InChIKey NUHCTOLBWMJMLX-UHFFFAOYSA-N Storage condition Store at RT. Stability Stable. Incompatible with strong oxidizing agents. Water Solubility slightly soluble |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available The above information is believed to be correct, but is not all-inclusive and should be used as a guide only. The information in this document is based on our current knowledge and is Correct safety instructions apply to this product. This information does not represent a warranty as to the properties of this product. See reverse side of invoice or packing slip. |
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