CAS Number4261-42-1
Synonyms(1S)-1,5-Anhydro-1-[2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxo-4H-chromen-6-yl]-D-glucitol; Lespecapitoside; luteolin-6-C-glucoside; Luteolin6-C-β-D-glucoside,Luteolin6-C-glucoside; 2-(3,4-Dihydroxyphenyl)-6-b-D-glucopyranosyl-5,7-dihydroxy-4H-1-benzopyran-4-one; 2-(3,4-Dihydroxyphenyl)-6-β-D-glucopyranosyl-5,7-dihydroxy-4H-1-benzopyran-4-one; Isoorientin; Homoorientin
Molecular FormulaC21H20O11
Molecular Weight448.38
Purity≥98 (HPLC)%
Density1.8±0.1 g/cm3
Boiling Point856.7±65.0 °C at 760 mmHg
Melting Point236 - 237 °C ( (457 - 459 °F))
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 4261-42-1 |
| Catalog No. | 2A-9010857 |
| Chinese Name | 异荭草苷; 异荭草素 |
| Synonyms | (1S)-1,5-Anhydro-1-[2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxo-4H-chromen-6-yl]-D-glucitol; Lespecapitoside; luteolin-6-C-glucoside; Luteolin6-C-β-D-glucoside,Luteolin6-C-glucoside; 2-(3,4-Dihydroxyphenyl)-6-b-D-glucopyranosyl-5,7-dihydroxy-4H-1-benzopyran-4-one; 2-(3,4-Dihydroxyphenyl)-6-β-D-glucopyranosyl-5,7-dihydroxy-4H-1-benzopyran-4-one; Isoorientin; Homoorientin |
| Molecular Formula | C21H20O11 |
| Molecular Weight | 448.38 |
| Purity | ≥98 (HPLC) |
| Density | 1.8±0.1 g/cm3 |
| Boiling Point | 856.7±65.0 °C at 760 mmHg |
| Melting Point | 236 - 237 °C ( (457 - 459 °F)) |
| Appearance | powder |
| Water Solubility | methanol: soluble5mg/mL, clear, colorless to yello |
| Storage Condition | 2-8°C |
| Application | Isoorientin is a potent COX-2 inhibitor with an IC50 value of 39 μM. |
| PubChem ID | 329815353 |
| MDL Number | MFCD00017433 |
| SMILES | OC[C@H]1O[C@H]([C@H](O)[C@@H](O)[C@@H]1O)c2c(O)cc3OC(=CC(=O)c3c2O)c4ccc(O)c(O)c4 |
| InChI | 1S/C21H20O11/c22-6-14-17(27)19(29)20(30)21(32-14)16-11(26)5-13-15(18(16)28)10(25)4-12(31-13)7-1-2-8(23)9(24)3-7/h1-5,14,17,19-24,26-30H,6H2/t14-,17-,19+,20-,21+/m1/s1 |
| InChI Key | ODBRNZZJSYPIDI-VJXVFPJBSA-N |
| NACRES Code | NA.25 |
| UNSPSC | 12352205 |
| Hazard Symbols | Transport |
| MSDS | msds/10857_1_4261_42_1.pdf |
| Bioactivity | Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM. Related Catalog Research Areas >> Cancer Natural Products >> Flavonoids Research Areas >> Inflammation/Immunology Target COX-2:39 μM (IC50) In Vitro Isoorientin is a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa[1]. PANC-1 and PATU-8988 cells are grown for 24 hours in the presence of Isoorientin (0, 20, 40, 80, and 160 μM), and a CCK8 solution is added. The cell viability decreases significantly at the concentrations of 20, 40, 80, and 160 μM. After the cells are cultured with Isoorientin (0, 20, 40, 80, and 160 μM for PANC-1; 0, 20, 40, 80, 160, and 320 μM for PATU-8988) for 24 hours, the expression of p-AMPK and AMPK is assessed by Western blotting. After the Isoorientin treatment, the p-AMPK expression is increased. Then, in the shRNA group, the concentration of 80 μM is used to detect the effects of Isoorientin. The expression levels of AMPK and p-AMPK are much lower in the shRNA group than in the wild-type PC cells (WT) and the group that is transfected with a negative control lentivirus (NC)[2]. In Vivo Animals treated with Isoorientin at 10 mg/kg and 20 mg/kg body weight have a statistically significant reduction in paw edema, with a mean peak thickness of 1.19±0.05 mm and 1.08±0.04 mm, respectively. This indicated that Isoorientin significantly attenuates paw edema compared with the control group[3]. Cell Assay PANC-1 and PATU-8988 cells are plated onto 96-well plates. Each well contain ~5,000 cells and 200 μL of the medium with 10% FBS. When the cells of each well reach 70% confluency, the medium is changed, and FBS-free medium with different concentrations of Isoorientin is added. After 24 hours, the cells are washed with PBS once, the medium containing Isoorientin is discarded, and 100 μL of FBS-free medium with 10 μL of the Cell Counting Kit 8 (CCK8) reagent are added. The cells are incubated for another 1-2 hours at 37°C, and the absorbance of each well is detected using an ELISA reader at 490 nm. Cell viability is expressed as the fold change of absorbance[2]. Animal Admin Mice[3] In the case of paw edema model the Isoorientin or Celecoxib is given intraperitoneally and Carrageenan is injected into the paw directly one hour later. In air pouch model all the treatments are given along with Carrageenan directly into the pouch cavity. Isoorientin is injected three hours earlier than injection of Carrageenan into the pouch cavity. Isoorientin and Celecoxib are administered into the mice air pouch. The stock solutions of Isoorientin (100 mg/mL) and Celecoxib (100 mg/mL) are prepared in DMSO and further dilutions are made at the time of treatments. Animals are divided, into 5 different groups as follows: control (DMSO treated); Carrageenan (0.5 mL of 1.5% (w/v) Carrageenan in saline) treated; Carrageenan+Celecoxib (20 mg/kg body weight) treated; Carrageenan+Isoorientin (10 mg/Kg body weight) treated; Carrageenan+Isoorientin (20 mg/Kg body weight) treated. References [1]. Sumalatha M, et al. Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa. Nat Prod Commun. 2015 Oct;10(10):1703-4. [2]. Ye T, et al. Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells. Onco Targets Ther. 2016 Dec 12;9:7481-7492. [3]. Anilkumar K, et al. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxid Med Cell Longev. 2017;2017:5498054. Chemical & Physical Properties Density 1.8±0.1 g/cm3 Boiling Point 856.7±65.0 °C at 760 mmHg Melting Point 245-246ºC Molecular Formula C21H20O11 Molecular Weight 448.377 Flash Point 303.2±27.8 °C Exact Mass 448.100555 PSA 201.28000 LogP 1.58 Appearance of Characters yellow Vapour Pressure 0.0±0.3 mmHg at 25°C Index of Refraction 1.767 InChIKey ODBRNZZJSYPIDI-VJXVFPJBSA-N SMILES O=c1cc(-c2ccc(O)c(O)c2)oc2cc(O)c(C3OC(CO)C(O)C(O)C3O)c(O)c12 Storage condition 2-8°C Water Solubility methanol: soluble5mg/mL, clear, colorless to yellow |
| Use of | Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM. Properties Articles33 Name Isoorientin Synonym More Synonyms Luteolin-6-C-glucoside Biological Activity Description Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM. Related Catalog Research Areas >> Cancer Natural Products >> Flavonoids Research Areas >> Inflammation/Immunology COX-2:39 μM (IC50) References [1]. Sumalatha M, et al. Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa. Nat Prod Commun. 2015 Oct;10(10):1703-4. [2]. Ye T, et al. Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells. Onco Targets Ther. 2016 Dec 12;9:7481-7492. [3]. Anilkumar K, et al. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxid Med Cell Longev. 2017;2017:5498054. Chemical & Physical Properties Molecular Formula C21H20O11 Exact Mass 448.100555 PSA 201.28000 Appearance of Characters yellow Index of Refraction 1.767 InChIKey ODBRNZZJSYPIDI-VJXVFPJBSA-N |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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