CAS Number3690-10-6
Synonyms4-Deoxyuridine; 1--D-Ribofuranosyl-2(1H)-pyrimidinone; 4-Deoyuridine; 2(1H)-PyriMidinone,1-b-D-ribofuranosyl; 1-(β-D-Ribofuranosyl)pyrimidin-2(1H)-one; Pyrimidin-2-one b-Ribofuranoside; Zebularine; 1-b-D-Ribofuranosyl-2(1H)-pyrimidinone; Pyrimidin-2-one-D-Ribofuranoside; 1-(β-D-Ribofuranosyl)-2(1H)-pyrimidinone
Molecular FormulaC9H12N2O5
Molecular Weight228.20
Purity98%
Density1.7±0.1 g/cm3
Boiling Point499.0±55.0 °C at 760 mmHg
Melting Point160-162?C
Appearancesolid
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 3690-10-6 |
| Catalog No. | 2A-9094127 |
| Chinese Name | 泽布拉林 |
| Synonyms | 4-Deoxyuridine; 1--D-Ribofuranosyl-2(1H)-pyrimidinone; 4-Deoyuridine; 2(1H)-PyriMidinone,1-b-D-ribofuranosyl; 1-(β-D-Ribofuranosyl)pyrimidin-2(1H)-one; Pyrimidin-2-one b-Ribofuranoside; Zebularine; 1-b-D-Ribofuranosyl-2(1H)-pyrimidinone; Pyrimidin-2-one-D-Ribofuranoside; 1-(β-D-Ribofuranosyl)-2(1H)-pyrimidinone |
| Molecular Formula | C9H12N2O5 |
| Molecular Weight | 228.20 |
| Purity | 98 |
| Density | 1.7±0.1 g/cm3 |
| Boiling Point | 499.0±55.0 °C at 760 mmHg |
| Melting Point | 160-162?C |
| Appearance | solid |
| Water Solubility | DMSO: 16 mg/mL, soluble |
| Storage Condition | 2-8°C |
| Application | Zebularine (NSC309132; 4-Deoxyuridine) is a DNA methyltransferase inhibitor and a cytidine deaminase inhibitor with a Ki value of 0.95 μM. |
| PubChem ID | 10231000 |
| SMILES | O=c1ncccn1C1OC(CO)C(O)C1O |
| InChI | InChI=1S/C9H12N2O5/c12-4-5-6(13)7(14)8(16-5)11-3-1-2-10-9(11)15/h1-3,5-8,12-14H,4H2 |
| InChI Key | RPQZTTQVRYEKCR-WCTZXXKLSA-N |
| Hazard Symbols | transportation |
| Bioactivity | Zebularine (NSC309132; 4-Deoxyuridine) is a DNA methyltransferase inhibitor; also an inhibitor of cytidine deaminase with a Ki of 0.95 μM. Related Catalog Signaling Pathways >> Autophagy >> Autophagy Signaling Pathways >> Epigenetics >> DNA Methyltransferase Research Areas >> Cancer Target DNMT1 DNMT3a/3L Cytidine deaminase:0.95 μM (Ki) Autophagy In Vitro Zebularine exerts its demethylation activity by stabilizing the binding of DNMTs to DNA, hindering the methylation and decreasing the dissociation, thereby trapping the enzyme and preventing turnover even at other sites. zebularine enhances tumor cell chemo- and radiosensitivity and has antimitogenic and angiostatic activities[1]. Zebularine inhibits DNA methylation and reactivates a gene previously silenced by methylation. Zebularine induces the myogenic phenotype in 10T1/2 cells, which is a phenomenon unique to DNA methylation inhibitors. Zebularine reactivates a silenced p16 gene and demethylated its promoter region in T24 bladder carcinoma cells[2]. Zebularine treatment inhibits cell growth in a dose and time dependent manner with an IC50 of ∼100 μM and 150 μM in MDA-MB-231 and MCF-7 cells, respectively, on 96 h exposure. At high doses zebularine induced changes in apoptotic proteins in a cell line specific manner manifested by alteration in caspase-3, Bax, Bcl2 and PARP cleavage[3]. Zebularine is also a potent competitive inhibitor of the enzyme CR deaminase. The Ki for zebularine is 0.95μM[4]. In Vivo Zebularine is only slightly cytotoxic to tumor-bearing mice. Compared with those in control mice, tumor volumes are statistically significantly reduced in mice treated with high-dose zebularine administered by intraperitoneal injection or by oral gavage[2]. Zebularine also enhances the survival time of mice with L1210 leukemia treated with 5-AZA-CdR. About 27% of the mice treated with this drug combination has a survival time longer than the mice treated with only 5-AZA-CdR[4]. Cell Assay For methylation analysis, 10T1/2 cells and T24 cells are treated with the various concentrations of zebularine. For 10T1/2 cells, the medium is changed 24 hours after the initial drug treatment, whereas for T24 cells, the medium is changed 24 hours or 48 hours after the initial drug treatment. DNA and RNA are harvested from 10T1/2 cells 72 hours after initial drug treatment and from T24 cells 96 hours after initial drug treatment. The methylation status of the indicated DNA regions is measured in two separate and independent experiments, both of which are done in duplicate[2]. Animal Admin EJ6 cells (5 × 105/injection) suspended in PBS are inoculated subcutaneously into the right and left flanks (along the midaxillary lines) of 4- to 6-week-old male BALB/c nu/nu mice. Mice (n=30) are randomLy divided into six groups (intraperitoneal control, oral control, intraperitoneal zebularine at 500 mg/kg, oral zebularine at 500 mg/kg, intraperitoneal zebularine at 1000 mg/kg, and oral zebularine at 1000 mg/kg). Each group consisted of five mice (at least six tumors per group; one or two mice per group are randomLy killed at earlier time points to establish a time course of expression). After 2-3 weeks and after macroscopic tumors (50-200 mm3) had formed, zebularine or control treatments are initiated. Zebularine, at doses of 500 mg/kg or 1000 mg/kg, is administered daily by intraperitoneal injection or oral gavage in a solution of 0.45% saline over a period of 18 days[2]. References [1]. Champion C, et al. Mechanistic insights on the inhibition of c5 DNA methyltransferases by zebularine. PLoS One. 2010 Aug 24;5(8):e12388. [2]. Cheng JC, et al. Inhibition of DNA methylation and reactivation of silenced genes by zebularine. J Natl Cancer Inst. 2003 Mar 5;95(5):399-409. [3]. Billam M, et al. Effects of a novel DNA methyltransferase inhibitor zebularine on human breast cancer cells. Breast Cancer Res Treat. 2010 Apr;120(3):581-92. [4]. Lemaire M, et al. Inhibition of cytidine deaminase by zebularine enhances the antineoplastic action of 5-aza-2'-deoxycytidine. Cancer Chemother Pharmacol. 2009 Feb;63(3):411-6. Chemical & Physical Properties Density 1.7±0.1 g/cm3 Boiling Point 499.0±55.0 °C at 760 mmHg Melting Point 160-162?C Molecular Formula C9H12N2O5 Molecular Weight 228.202 Flash Point 255.6±31.5 °C Exact Mass 228.074615 PSA 104.81000 LogP -1.31 Appearance of Characters solid Vapour Pressure 0.0±2.9 mmHg at 25°C Index of Refraction 1.697 InChIKey RPQZTTQVRYEKCR-WCTZXXKLSA-N SMILES O=c1ncccn1C1OC(CO)C(O)C1O Storage condition 2-8°C Water Solubility DMSO: 16 mg/mL, soluble |
| Use of | Zebularine (NSC309132; 4-Deoxyuridine) is a DNA methyltransferase inhibitor; also an inhibitor of cytidine deaminase with a Ki of 0.95 μM. Properties Articles20 Name zebularine Synonym More Synonyms Zebularine Biological Activity Description Zebularine (NSC309132; 4-Deoxyuridine) is a DNA methyltransferase inhibitor; also an inhibitor of cytidine deaminase with a Ki of 0.95 μM. Related Catalog Signaling Pathways >> Autophagy >> Autophagy Signaling Pathways >> Epigenetics >> DNA Methyltransferase Research Areas >> Cancer Cytidine deaminase:0.95 μM (Ki) References [1]. Champion C, et al. Mechanistic insights on the inhibition of c5 DNA methyltransferases by zebularine. PLoS One. 2010 Aug 24;5(8):e12388. [2]. Cheng JC, et al. Inhibition of DNA methylation and reactivation of silenced genes by zebularine. J Natl Cancer Inst. 2003 Mar 5;95(5):399-409. [3]. Billam M, et al. Effects of a novel DNA methyltransferase inhibitor zebularine on human breast cancer cells. Breast Cancer Res Treat. 2010 Apr;120(3):581-92. [4]. Lemaire M, et al. Inhibition of cytidine deaminase by zebularine enhances the antineoplastic action of 5-aza-2'-deoxycytidine. Cancer Chemother Pharmacol. 2009 Feb;63(3):411-6. Chemical & Physical Properties Molecular Formula C9H12N2O5 Exact Mass 228.074615 PSA 104.81000 LogP -1.31 Appearance of Characters solid Index of Refraction 1.697 InChIKey RPQZTTQVRYEKCR-WCTZXXKLSA-N |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 Names specified by the United Nations (UN) European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Dangerous Regulations: No International Maritime Transport Dangerous Regulations Maritime Pollutants: No International Air Transport Risk Regulations: No 14.6 Special reminder to users No data available |
| Related Products in Specialty Chemicals | ||
|---|---|---|
| ammonium dibutyl phosphate | 38491-08-6 | 2A-9094120 |
| 5,5-DIBROMOMELDRUM'S ACID | 66131-14-4 | 2A-9094121 |
| Tiopronin | 1953-02-2 | 2A-9094122 |
| Tiopronin | 6740-88-1 | 2A-9094123 |
| l-Ketamine | 33643-46-8 | 2A-9094124 |
| 4-[2-(3,4-DIHYDRO-6,7-DIMETHOXY-2(1H)-ISOQUINOLINYL)ETHYL]-BENZENAMINE | 82925-02-8 | 2A-9094130 |
| BenzaMide, N-[4-[2-(3,4-dihydro-6,7-diMethoxy-2(1H)-isoquinolinyl)ethyl]phenyl]-4,5-diMethoxy-2-nitro- | 1024592-54-8 | 2A-9094131 |
| METHYLIONONE | 1335-46-2 | 2A-9094135 |
| (Z)-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one | 52340-45-1 | 2A-9094137 |
| (Z)-4-(2,6,6-trimethyl-2-cyclohexen-1-yl)-3-buten-2-one | 1914946-33-0 | 2A-9094138 |
| Browse all Specialty Chemicals products » | ||
Phone:
630-322-8886
630-322-8887
Email:
Office Locations:
Chicago, IL, USA
San Francisco, CA, USA
EN
SDS