CAS Number114162-64-0
SynonymsX-Gluc CHA Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate; Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate; X-glucuronide CHA salt; X-gluc/X-GlcA; X-Glu,CHA Salt; 5-Bromo-4-chlo; X-GlcA CHA Salt; Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1); 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt; X-GlcA,X-glucuronide CHA salt; 5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E; 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt; 5-BROMO-4-CHLORO-3
Molecular FormulaC20H26BrClN2O7
Molecular Weight521.79
Purity≥98 (TLC)%
Density1.4241 (rough estimate)
Boiling Point732.2ºC at 760 mmHg
Melting Point228-230ºC
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 114162-64-0 |
| Catalog No. | 2A-9050813 |
| Chinese Name | 5-溴-4-氯-3-吲哚基-β-D-葡糖苷酸环己胺盐(X-GlcA) |
| Synonyms | X-Gluc CHA Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate; Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate; X-glucuronide CHA salt; X-gluc/X-GlcA; X-Glu,CHA Salt; 5-Bromo-4-chlo; X-GlcA CHA Salt; Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1); 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt; X-GlcA,X-glucuronide CHA salt; 5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E; 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt; 5-BROMO-4-CHLORO-3 |
| Molecular Formula | C20H26BrClN2O7 |
| Molecular Weight | 521.79 |
| Purity | ≥98 (TLC) |
| Density | 1.4241 (rough estimate) |
| Boiling Point | 732.2ºC at 760 mmHg |
| Melting Point | 228-230ºC |
| Appearance | powder |
| Water Solubility | DMF: soluble |
| Storage Condition | 2-8°C |
| Application | X-Gluc Cyclohexylamine is a dye reagent used to detect beta-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexylamine can be used to detect E. coli contamination in food, water and ur |
| HTC | 29349990 |
| PubChem ID | 24891926 |
| MDL Number | MFCD00058543 |
| SMILES | NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34 |
| InChI | 1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1 |
| InChI Key | JXCKZXHCJOVIAV-CYRSAHDMSA-N |
| NACRES Code | NA.85 |
| UNSPSC | 12352200 |
| Hazard Symbols | transportation |
| MSDS | msds/50813_1_114162_64_0.pdf |
| Bioactivity | X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation. References [1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. Chemical & Physical Properties Density 1.4241 (rough estimate) Boiling Point 732.2ºC at 760 mmHg Melting Point 228-230ºC Molecular Formula C20H26BrClN2O7 Molecular Weight 521.787 Flash Point 396.6ºC Exact Mass 520.061157 PSA 158.26000 LogP 2.83290 Vapour Pressure 1.66E-22mmHg at 25°C Index of Refraction 1.6110 (estimate) InChIKey JXCKZXHCJOVIAV-CYRSAHDMSA-N SMILES NC1CCCCC1.O=C(O)C1OC(Oc2c[nH]c3ccc(Br)c(Cl)c23)C(O)C(O)C1O Storage condition 2-8°C Water Solubility DMF: soluble |
| Use of | X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1]. Properties Articles13 Name 5-bromo-4-chloro-3-indolyl b-d Synonym More Synonyms X-glucuronide CHA salt Biological Activity Description X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others References [1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. Chemical & Physical Properties Molecular Formula C20H26BrClN2O7 Exact Mass 520.061157 PSA 158.26000 LogP 2.83290 Index of Refraction 1.6110 (estimate) InChIKey JXCKZXHCJOVIAV-CYRSAHDMSA-N Water Solubility DMF: soluble |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available See reverse side of invoice or packing slip. |
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