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Basic Orange 14 structure, CAS 10127-02-3

Basic Orange 14

CAS Number10127-02-3

SynonymsN,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride; Acridine Orange hemi(zinc chloride) salt; MFCD00081043; zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride; Acridine Orange; N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2); Euchrysin GNY; EINECS 200-614-0

Molecular FormulaC17H20ClN3 · HCl · 1/2ZnCl2

Molecular Weight369.96

Purity98.00%

Density

Boiling Point468.6ºC at 760 mmHg

Melting Point165 °C

Flash Point

Appearancebrown powder

EINECS

MSDSChineseEnglish

Symbol6.1

Signal WordWarning

Cat. No.: 2A-9034666 Purity: 98.00%
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Quality Control of [ 10127-02-3 ] Purity: 98.00% SDS Specification MoL

COA NMR CNMR HPLC LC-MS
Chemical & Physical Properties
CAS Number10127-02-3
Catalog No.2A-9034666
Chinese Name碱性橙 14
SynonymsN,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride; Acridine Orange hemi(zinc chloride) salt; MFCD00081043; zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride; Acridine Orange; N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2); Euchrysin GNY; EINECS 200-614-0
Molecular FormulaC17H20ClN3 · HCl · 1/2ZnCl2
Molecular Weight369.96
Purity98.00
Boiling Point468.6ºC at 760 mmHg
Melting Point165 °C
Appearancebrown powder
Water Solubilitysoluble
Storage ConditionStore sealed and dry.
PackagingI; II; III
ApplicationAcridine orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrating nucleic acid-selective fluorescent dye. Acridine orange zinc chloride salt produces orange fluorescence when combined with ssD
HTC32041300
PubChem ID24849765
SMILESCN(C)c1ccc2cc3ccc(N(C)C)cc3nc2c1.Cl.Cl[Zn]Cl
InChIInChI=1S/C17H19N3.3ClH.Zn/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;/h5-11H,1-4H3;3*1H;/q;;;;+2/p-2
InChI KeyVADJQOXWNSPOQA-UHFFFAOYSA-L
Hazard Symbols6.1
BioactivityAcridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3]. Related Catalog Research Areas >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. Stain cells with Acridine Orange zinc chloride salt (1 μM; 20 minutes; 37 ℃). 2. Wash cells with PBS. 3. Observe cells by confocal laser scanning microscopy (FV3000, Olympus). In Vivo Acridine Orange (0.1 mg/kg, IV, dog) zinc chloride salt showed no signs of clinical toxicity over 30 days and no abnormalities were found in the blood [1]. References [1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56. [3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925. [4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172. Chemical & Physical Properties Boiling Point 468.6ºC at 760 mmHg Melting Point 165 °C Molecular Formula C34H40Cl4N6Zn Molecular Weight 739.943 Flash Point 237.2ºC Exact Mass 736.135986 PSA 38.74000 LogP 10.02050 InChIKey VADJQOXWNSPOQA-UHFFFAOYSA-L SMILES CN(C)c1ccc2cc3ccc(N(C)C)cc3nc2c1.Cl.Cl[Zn]Cl Storage condition Store at RT. Water Solubility soluble
Use ofProperties Articles17 Name Basic Orange 14 Synonym More Synonyms Basic Orange 14 Biological Activity Description Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3]. Related Catalog Research Areas >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. Stain cells with Acridine Orange zinc chloride salt (1 μM; 20 minutes; 37 ℃). 2. Wash cells with PBS. 3. Observe cells by confocal laser scanning microscopy (FV3000, Olympus). References [1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56. [3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925. [4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172. Chemical & Physical Properties Exact Mass 736.135986 PSA 38.74000 LogP 10.02050 InChIKey VADJQOXWNSPOQA-UHFFFAOYSA-L Storage condition Store at RT. Water Solubility soluble
Transport InfoProduct name: Purity: 98.0%
MSDS Transport InfoModule 14. Shipping information United Nations classification: inconsistent with United Nations classification standards UN number: not specified Alkaline Orange 14
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