CAS Number333338-18-4
SynonymsMFCD00066288; di-Sodium 4-nitrophenyl phosphate; pNPP disodium salt hexahydrate; DisodiuM 4-Nitrophenylphosphate Hexahydrate; Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt Hexahydrate; Phosphatase substrate; 4-Nitrophenyl Phosphate,Disodium Salt Hexahydrate; p-Nitrophenyl phosphate disodium hexahydrate; pNPP disodium hexahydrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di; Sodium 4-nitrophenyl phosphate hexahydrate; EINECS 224-246-5; Disodium 4-Nitrophenyl Phosphate Hexahydrate
Molecular FormulaO2NC6H4OP(O)(ONa)2 · 6H2O
Molecular Weight371.14
Boiling Point457.8ºC at 760mmHg
Melting Point>300ºC(lit.)
Appearancetablets | white to pale yellow
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 333338-18-4 |
| Catalog No. | 2A-1184642 |
| Chinese Name | 4-硝基苯磷酸盐 二钠盐 六水合物(PNPP) |
| Synonyms | MFCD00066288; di-Sodium 4-nitrophenyl phosphate; pNPP disodium salt hexahydrate; DisodiuM 4-Nitrophenylphosphate Hexahydrate; Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt Hexahydrate; Phosphatase substrate; 4-Nitrophenyl Phosphate,Disodium Salt Hexahydrate; p-Nitrophenyl phosphate disodium hexahydrate; pNPP disodium hexahydrate; 4-Nitrophenyl phosphate disodium salt hexahydrate; p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di; Sodium 4-nitrophenyl phosphate hexahydrate; EINECS 224-246-5; Disodium 4-Nitrophenyl Phosphate Hexahydrate |
| Molecular Formula | O2NC6H4OP(O)(ONa)2 · 6H2O |
| Molecular Weight | 371.14 |
| Boiling Point | 457.8ºC at 760mmHg |
| Melting Point | >300ºC(lit.) |
| Appearance | tablets | white to pale yellow |
| Water Solubility | H2O: 25 mg/mL at 20 °C, clear, yellow-green |
| Storage Condition | 2-8°C |
| Application | Disodium 4-nitrophenylphosphate (p-nitrophenylphosphate) hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in protein tyrosine phosphatase activity assays. 4-Nitrophenylpho |
| PubChem ID | 24897580 |
| InChI | InChI=1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2 |
| InChI Key | KAKKHKRHCKCAGH-UHFFFAOYSA-L |
| Hazard Symbols | transportation |
| Bioactivity | 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro 4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2]. References [1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205. [2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877. Chemical & Physical Properties Boiling Point 457.8ºC at 760mmHg Melting Point >300ºC(lit.) Molecular Formula C6H16NNa2O12P Molecular Weight 371.14400 Flash Point 230.7ºC Exact Mass 371.02100 PSA 183.43000 LogP 2.08010 Appearance of Characters tablets | white to pale yellow Storage condition 2-8°C Water Solubility H2O: 25 mg/mL at 20 °C, clear, yellow-green |
| Use of | 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1]. Properties Articles164 Name disodium,(4-nitrophenyl) phosphate,hexahydrate Synonym More Synonyms Biological Activity Description 4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro 4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2]. References [2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877. Chemical & Physical Properties Molecular Formula C6H16NNa2O12P Exact Mass 371.02100 PSA 183.43000 LogP 2.08010 Appearance of Characters tablets | white to pale yellow |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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