CAS Number92-32-0
SynonymsN-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methylmethanaminium chloride; Pyronine Y; Tetramethyl pyronin; EINECS 202-147-8; Ammonium, [6-(dimethylamino)-3H-xanthen-3-ylidene]dimethyl-, chloride; N-[6-(Dimethylamino)-3H-xanthene-3-ylidene]-N-methylmethanaminium Chloride; Pyronine; Methanaminium, N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methyl-, chloride (1:1); Pyroniny(G); Methyl pyronin; Pyronin G; Pyronine G chloride; Pyronine ZH; MFCD00011725; [6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium,chloride; pyronine G hydrochloride; xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1); Xanthylium, 3,6-bis(dimethylamino)-, chloride; Pyronin Y; 6-(Dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium chloride; Pyronine G; Pyronin Yellow; 3,6-Bis(dimethylamino)xanthenium chloride; [6-(Dimethy
Molecular FormulaC17H19ClN2O
Molecular Weight302.80
Purity98.00%
Melting Point250-260 °C (lit.)
Appearancepowder
EINECS202-147-8
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 92-32-0 |
| Catalog No. | 2A-9017743 |
| Chinese Name | 派洛宁Y |
| Synonyms | N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methylmethanaminium chloride; Pyronine Y; Tetramethyl pyronin; EINECS 202-147-8; Ammonium, [6-(dimethylamino)-3H-xanthen-3-ylidene]dimethyl-, chloride; N-[6-(Dimethylamino)-3H-xanthene-3-ylidene]-N-methylmethanaminium Chloride; Pyronine; Methanaminium, N-[6-(dimethylamino)-3H-xanthen-3-ylidene]-N-methyl-, chloride (1:1); Pyroniny(G); Methyl pyronin; Pyronin G; Pyronine G chloride; Pyronine ZH; MFCD00011725; [6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium,chloride; pyronine G hydrochloride; xanthylium, 3,6-bis(dimethylamino)-, chloride (1:1); Xanthylium, 3,6-bis(dimethylamino)-, chloride; Pyronin Y; 6-(Dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium chloride; Pyronine G; Pyronin Yellow; 3,6-Bis(dimethylamino)xanthenium chloride; [6-(Dimethy |
| Molecular Formula | C17H19ClN2O |
| Molecular Weight | 302.80 |
| Purity | 98.00 |
| Melting Point | 250-260 °C (lit.) |
| Appearance | powder |
| Water Solubility | Soluble in water; sparingly soluble in ethanol, et |
| Storage Condition | This product should be stored in a sealed, cool, d |
| Application | Pyronin Y is an intercalating cationic dye specific for RNA. |
| EINECS | 202-147-8 |
| HTC | 2921199090 |
| PubChem ID | 57654569 |
| MDL Number | MFCD00011725 |
| SMILES | [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C |
| InChI | 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1 |
| InChI Key | INCIMLINXXICKS-UHFFFAOYSA-M |
| Beilstein/REAXYS | 3795789 |
| NACRES Code | NA.52 |
| UNSPSC | 12171500 |
| Hazard Symbols | Transport |
| MSDS | msds/17743_1_92_32_0.pdf |
| Bioactivity | Pyronin Y is an intercalating cationic dye that shows specificity towards RNA. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin[1]. A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting[2]. In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells[3]. Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent[4]. Cell Assay The cells are resuspended and stained for 30 min in 1.0 mL of 0.01% pyronin Y in sodiumacetate buffer (1.0 M, pH 4.7). For this purpose 1.0 g of pyronin Y is dissolved in 100 mL distilled water and this solution is purified by chloroform extraction (three fractions of 100 mL). The purified pyronin Y solution is diluted with 1.0 M sodiumacetate buffer pH 4.7 to the appropriate concentration and filtered through paper filters before use. Stained cell samples are studied by fluorescence microscopy (excitation filters SP 560 and LP 515, chromatic beam splitter at 580 nm, barrier filter LP 580) or by flow cytometry[2]. References [1]. Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35. [2]. Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20. [3]. Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46(11):5760-6. [4]. Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34(6-7):299-303. Chemical & Physical Properties Melting Point 250-260ºC(lit.) Molecular Formula C17H19ClN2O Molecular Weight 302.799 Exact Mass 302.118591 PSA 19.39000 InChIKey INCIMLINXXICKS-UHFFFAOYSA-M SMILES CN(C)c1ccc2cc3ccc(=[N+](C)C)cc-3oc2c1.[Cl-] Storage condition Store at RT. Water Solubility Soluble in water; sparingly soluble in ethanol, ethylene glycol, methyl cellosolve | Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL). |
| Use of | Pyronin Y is an intercalating cationic dye that shows specificity towards RNA. Properties Articles60 Name acridine red 3B Synonym More Synonyms Pyronin Y Biological Activity Description Pyronin Y is an intercalating cationic dye that shows specificity towards RNA. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others References [1]. Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35. [2]. Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20. [3]. Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46(11):5760-6. [4]. Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34(6-7):299-303. Chemical & Physical Properties Exact Mass 302.118591 PSA 19.39000 InChIKey INCIMLINXXICKS-UHFFFAOYSA-M Storage condition Store at RT. |
| Tax Rebate | 9.0% |
| Supervision | none |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 Names specified by the United Nations (UN) European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Dangerous Regulations: No International Maritime Transport Dangerous Regulations Maritime Pollutants: No International Air Transport Risk Regulations: No 14.6 Special reminder to users No data available |
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