CAS Number138182-21-5
SynonymsSalmon-GalRose-Gal; SALMON GALACTOSIDE; ROSE-GAL; SALMON-GAL; SALMON GLUCOSIDE; SALMON GLU; ROSE(TM)-GAL; ROSE(TM)-GLUCOSIDE; MFCD00467206; RED-GAL(R)
Molecular FormulaC14H16ClNO6
Molecular Weight329.733
Purity96+%
Density1.6±0.1 g/cm3
Boiling Point630.2±55.0 °C at 760 mmHg
AppearanceSolid;White to Light yellow powder to crystal
EINECS0
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 138182-21-5 |
| Catalog No. | 2A-1095371 |
| Chinese Name | 6-氯-3-吲哚-β-D-半乳糖皮蒽(约含13%乙酸乙酯)[用于生化研究] |
| Synonyms | Salmon-GalRose-Gal; SALMON GALACTOSIDE; ROSE-GAL; SALMON-GAL; SALMON GLUCOSIDE; SALMON GLU; ROSE(TM)-GAL; ROSE(TM)-GLUCOSIDE; MFCD00467206; RED-GAL(R) |
| Molecular Formula | C14H16ClNO6 |
| Molecular Weight | 329.733 |
| Purity | 96+ |
| Density | 1.6±0.1 g/cm3 |
| Boiling Point | 630.2±55.0 °C at 760 mmHg |
| Appearance | Solid;White to Light yellow powder to crystal |
| Storage Condition | Sealed, store at 2 ºC -8 ºC |
| Application | Rose-β-D-Gal is a fluorescent dye and a β-galactosidase substrate. Rose-β-D-Gal produces a pink/magenta color after reaction and has been used to detect β-Gal activity [1][2]. |
| EINECS | 0 |
| HTC | 0 |
| UN(IATA) | 0 |
| PubChem ID | 14899759 |
| SMILES | OCC1OC(Oc2c[nH]c3cc(Cl)ccc23)C(O)C(O)C1O |
| InChI | InChI=1S/C14H16ClNO6/c15-6-1-2-7-8(3-6)16-4-9(7)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1 |
| InChI Key | OQWBAXBVBGNSPW-MBJXGIAVSA-N |
| Bioactivity | Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Immunostaining of Tissues[1][2]: 1.Prepare 25 mg/mL Rose-β-D-Gal stock solution in Dimethyl Sulfoxide (DMSO) and store at -20 °C. It is vital to protect the stock solution from the light. 2.Prepare rinse buffer for Rose-β-D-Gal staining: 0.1 % Sodium Deoxycholate, 0.2 % IGEPAL CA-630, 2 mM MgCl2 in 0.1 M Na Phosphate buffer (pH 7.3). Prepare 1,000 mL of 0.5 M Na Phosphate buffer (pH 7.3) by mixing with 158 mL of 1 M NaH2PO4, 342 mL of 1 M Na 2HPO4, and 500 mL water. 3.Prepare substrate solution for Rose-β-D-Gal fresh, containing 1 mg/mL Rose-β-D-Gal, 5 mM Potassium Ferricyanide, and 5 mM Potassium Ferrocyanide in the rinse buffer. 4.Rehydrate frozen tissue sections in PBS: quickly wash with PBS twice, wash with rinse buffer for 10 min 3 time. 5.Expose to β-galactosidase substrate Rose-β-D-Gal: stain at 37 ℃ for as long as needed to see stain, up to an overnight time period, keep covered and in the dark during color development. Wash with PBS for 5 min twice. 6.Fix tissue in 4% PFA, unless on intend to continue with in situ hybridizations, and continue to step 4. Note: Rose-β-D-Gal is not very stable in alcohols or organic solvents, so must use water-based counterstains such as Gill's hematoxylin and cover slipping with aqueous mountant. References [1]. Ismail J A , et al. Immunohistologic labeling of murine endothelium[J]. 2003, 12(2):0-90. [2]. Komatsu Y, et al. In situ hybridization methods for mouse whole mounts and tissue sections with and without additional β-galactosidase staining. Methods Mol Biol. 2014;1092:1-15. Chemical & Physical Properties Density 1.6±0.1 g/cm3 Boiling Point 630.2±55.0 °C at 760 mmHg Molecular Formula C14H16ClNO6 Molecular Weight 329.733 Flash Point 335.0±31.5 °C Exact Mass 329.066620 PSA 115.17000 LogP -0.15 Vapour Pressure 0.0±1.9 mmHg at 25°C Index of Refraction 1.717 InChIKey OQWBAXBVBGNSPW-MBJXGIAVSA-N SMILES OCC1OC(Oc2c[nH]c3cc(Cl)ccc23)C(O)C(O)C1O Storage condition 2-8°C |
| Use of | Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2]. Properties Name 6-Chloro-1H-indol-3-yl-β-D-galactopyranoside Synonym More Synonyms Biological Activity Description Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others References [1]. Ismail J A , et al. Immunohistologic labeling of murine endothelium[J]. 2003, 12(2):0-90. [2]. Komatsu Y, et al. In situ hybridization methods for mouse whole mounts and tissue sections with and without additional β-galactosidase staining. Methods Mol Biol. 2014;1092:1-15. Chemical & Physical Properties Exact Mass 329.066620 PSA 115.17000 LogP -0.15 Index of Refraction 1.717 InChIKey OQWBAXBVBGNSPW-MBJXGIAVSA-N |
| Transport Info | Product name: 6-chloro-3-indole-β-D-galactanthracene (containing approximately 13% ethyl acetate) [for biochemical research] |
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