CAS Number129541-41-9
Synonyms5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Sodium Salt; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate; BC-Indicator,X-GlcA,X-glucuronide; X-gluc/X-GlcA; X-Glu,CHA Salt; X-Gluc Sodium Salt; Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1); X-GlcA,X-glucuronide CHA salt; 5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt; 5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E; 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt; 5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S.; X-Gluc CHA Salt; X-GlcA Sodium Salt; 5-Bromo-4-chloro-3-indolyl-β-
Molecular FormulaC20H26BrClN2O7
Molecular Weight444.59
Purity≥98%
Boiling Point732.2ºC at 760mmHg
Melting Point228-230°C
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 129541-41-9 |
| Catalog No. | 2A-9051975 |
| Chinese Name | 5-溴-4-氯-3-吲哚基β-D-葡萄糖苷酸钠盐 |
| Synonyms | 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Sodium Salt; 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt; 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate; BC-Indicator,X-GlcA,X-glucuronide; X-gluc/X-GlcA; X-Glu,CHA Salt; X-Gluc Sodium Salt; Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1); X-GlcA,X-glucuronide CHA salt; 5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt; 5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E; 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt; 5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S.; X-Gluc CHA Salt; X-GlcA Sodium Salt; 5-Bromo-4-chloro-3-indolyl-β- |
| Molecular Formula | C20H26BrClN2O7 |
| Molecular Weight | 444.59 |
| Purity | ≥98 |
| Boiling Point | 732.2ºC at 760mmHg |
| Melting Point | 228-230°C |
| Appearance | powder |
| Storage Condition | Seal and store at -20ºC |
| Application | Sodium X-Gluc is a dye reagent that detects beta-glucuronidase, an enzyme produced by E. coli. X-Monosodium Glutamate can be used to detect E. coli contamination in food, water and the urinary tract. |
| HTC | 29339990 |
| PubChem ID | 24891832 |
| MDL Number | MFCD00135782 |
| SMILES | [Na+].O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C([O-])=O)Oc2c[nH]c3ccc(Br)c(Cl)c23 |
| InChI | 1S/C14H13BrClNO7.Na/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;/h1-3,9-12,14,17-20H,(H,21,22);/q;+1/p-1/t9-,10-,11+,12-,14+;/m0./s1 |
| InChI Key | IBLSVGDGSKUDCT-CYRSAHDMSA-M |
| NACRES Code | NA.83 |
| UNSPSC | 12352204 |
| Hazard Symbols | transportation |
| MSDS | msds/51975_1_129541_41_9.pdf |
| Bioactivity | X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation. References [1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. Chemical & Physical Properties Boiling Point 732.2ºC at 760mmHg Melting Point 228-230°C Molecular Formula C20H26BrClN2O7 Molecular Weight 521.787 Flash Point 396.6ºC Exact Mass 520.061157 PSA 135.07000 Storage condition −20°C |
| Use of | X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1]. Properties Articles11 Name 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt Synonym More Synonyms Biological Activity Description X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others References [1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. Chemical & Physical Properties Molecular Formula C20H26BrClN2O7 Exact Mass 520.061157 PSA 135.07000 |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 Names specified by the United Nations (UN) European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Dangerous Regulations: No International Maritime Transport Dangerous Regulations Maritime Pollutants: No International Air Transport Risk Regulations: No 14.6 Special reminder to users No data available See reverse side of invoice or packing slip. |
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