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| Chemical & Physical Properties | |
|---|---|
| CAS Number | 108964-32-5 |
| Catalog No. | 2A-9050429 |
| Chinese Name | 荧光钙探针 Fura 2-AM |
| Synonyms | Fura 2am; Fura-2 pentakis(acetoxymethyl) ester; MFCD00036976; Fura-2 AM |
| Molecular Formula | C44H47N3O24 |
| Molecular Weight | 1001.85 |
| Purity | ≥95.0 (HPLC) |
| Density | 1.4±0.1 g/cm3 |
| Boiling Point | 975.9±75.0 °C at 760 mmHg |
| Appearance | solid |
| Water Solubility | DMSO: soluble |
| Storage Condition | Sealed in a cool, dry environment at -20 ºC |
| Application | Fura-2 AM is a ratiometric fluorescent Ca2+ indicator. |
| PubChem ID | 57651141 |
| MDL Number | MFCD00036976 |
| SMILES | CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O |
| InChI | 1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3 |
| InChI Key | VPSRLGDRGCKUTK-UHFFFAOYSA-N |
| Beilstein/REAXYS | 8183748 |
| NACRES Code | NA.32 |
| UNSPSC | 12171500 |
| MSDS | msds/50429_1_108964_32_5.pdf |
| Bioactivity | Fura-2 AM is a ratiometric fluorescent Ca2+ indicator. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro The trypsin (100 nM) mediated Ca2+ transients are imagined in tsA-201 cells loaded with either 750 nM (n=111), 500 nM (n=119) or 250 nM (n=130) Fura-2 AM and compare the average Ca2+ increase above the baseline to the value obtained in the initial experiments using 1 μM of Fura-2 AM. The Ca2+ increases above the baseline for the 750, 500 and 250 nM are 633.0±33.9 nM (n=111, P>0.05 compare to 1 μM Fura-2 AM), 552.6±22.7 nM (n=119, P>0.05 compare to 1 μM Fura-2 AM) and 616.0±24.9 nM (n=130, P>0.05 compare to 1 μM Fura-2 AM), respectively[1]. Cell Assay Cells are loaded with Fura-2 AM, which diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. Aliquot 2-mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1 µM Fura-2 AM solution. Vortex the solution vigorously for 1 minute. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Prepare a 35 mm dish containing 2-mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. Mount the coverslip on the imaging chamber[2]. References [1]. Tinning PW, et al. A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. J Microsc. 2017 Aug 24. [2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067. Chemical & Physical Properties Density 1.4±0.1 g/cm3 Boiling Point 975.9±75.0 °C at 760 mmHg Molecular Formula C44H47N3O24 Molecular Weight 1001.850 Flash Point 544.0±37.1 °C Exact Mass 1001.254944 PSA 327.11000 LogP 2.90 Vapour Pressure 0.0±0.3 mmHg at 25°C Index of Refraction 1.567 InChIKey VPSRLGDRGCKUTK-UHFFFAOYSA-N SMILES CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc1cc2cc(-c3ncc(C(=O)OCOC(C)=O)o3)oc2cc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O Storage condition −20°C Water Solubility DMSO: soluble |
| Use of | Fura-2 AM is a ratiometric fluorescent Ca2+ indicator. Properties Articles49 Name acetyloxymethyl 2-[5-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-4-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate Synonym More Synonyms Fura-2, AM Biological Activity Description Fura-2 AM is a ratiometric fluorescent Ca2+ indicator. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others References [2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067. Chemical & Physical Properties Molecular Formula C44H47N3O24 Exact Mass 1001.254944 PSA 327.11000 Index of Refraction 1.567 InChIKey VPSRLGDRGCKUTK-UHFFFAOYSA-N Water Solubility DMSO: soluble |
| Transport Info | Product Name: Fura-2, AM |
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