CAS Number63368-54-7
SynonymsN-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)-2-iodoacetamide; 4(5)-(Iodoacetamido)fluorescein
Molecular FormulaC22H14INO6
Molecular Weight515.25
Purity≥90 (HPLC)%
Density1.95 g/cm3
Boiling Point788.4ºC at 760 mmHg
Melting Point265-267ºC (dec.)(lit.)
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 63368-54-7 |
| Catalog No. | 2A-9045898 |
| Chinese Name | 5-碘乙酰氨基荧光素 |
| Synonyms | N-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)-2-iodoacetamide; 4(5)-(Iodoacetamido)fluorescein |
| Molecular Formula | C22H14INO6 |
| Molecular Weight | 515.25 |
| Purity | ≥90 (HPLC) |
| Density | 1.95 g/cm3 |
| Boiling Point | 788.4ºC at 760 mmHg |
| Melting Point | 265-267ºC (dec.)(lit.) |
| Appearance | powder |
| Storage Condition | Sealed in a cool, dry environment at -20 ºC |
| Application | 5-IAF is an azoacetamide derivative of fluorescein. |
| PubChem ID | 329815467 |
| SMILES | Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1 |
| InChI | 1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27) |
| InChI Key | UATCLPJEZJKNHE-UHFFFAOYSA-N |
| Beilstein/REAXYS | 6543244 |
| NACRES Code | NA.47 |
| UNSPSC | 12171500 |
| Hazard Symbols | transportation |
| MSDS | msds/45898_1_63368_54_7.pdf |
| Bioactivity | 5-IAF is an idoacetamide derivate of fluoresceine. Related Catalog Signaling Pathways >> Others >> Others Research Areas >> Cancer Dye Reagents In Vitro 8 µM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 µM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 µM 5-IAF[2]. Kinase Assay HepG2 cells are lysed in 1 mL 2% acetonitrile in water, and transfered to a 1.5 mL centrifuge tube. An additional 200 µL 2% acetonitrile is used to rinse the dish and combined with the cell extract. To measure the free GSH concentration, three aliquots of 80 µL cell extract are immediately transferred to 0.5 mL centrifuge tubes after a quick mix. Then 20 µL 50 µM NAC is added, followed by the addition of 100 µL 400 µM 5-IAF to the mixture. The derivatization is conducted at room temperature in dark for 1 h, after which the mixtures are centrifuged at 9000× g for 5 min to sediment the insoluble proteins. 10 µL supernatant is diluted 100× with water and analyzed immediately by CE-LIF[2]. References [1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7. Chemical & Physical Properties Density 1.95 g/cm3 Boiling Point 788.4ºC at 760 mmHg Melting Point 265-267ºC (dec.)(lit.) Molecular Formula C22H14INO6 Molecular Weight 515.25400 Flash Point 430.6ºC Exact Mass 514.98700 PSA 116.84000 LogP 4.41510 Index of Refraction 1.781 InChIKey UATCLPJEZJKNHE-UHFFFAOYSA-N SMILES O=C(CI)Nc1ccc2c(c1)C(=O)OC21c2ccc(O)cc2Oc2cc(O)ccc21 |
| Use of | 5-IAF is an idoacetamide derivate of fluoresceine. Properties Articles30 Name 5-(Iodoacetamido)fluorescein Synonym More Synonyms 5-(Iodoacetamido)fluorescein Biological Activity Description 5-IAF is an idoacetamide derivate of fluoresceine. Related Catalog Signaling Pathways >> Others >> Others Research Areas >> Cancer Dye Reagents In Vitro 8 µM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 µM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 µM 5-IAF[2]. References [1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. Chemical & Physical Properties Molecular Formula C22H14INO6 Exact Mass 514.98700 PSA 116.84000 LogP 4.41510 Index of Refraction 1.781 InChIKey UATCLPJEZJKNHE-UHFFFAOYSA-N |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 United Nations number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special precautions No data available |
| Related Products in Specialty Chemicals | ||
|---|---|---|
| Nuarimol | 63284-71-9 | 2A-9045887 |
| Iron(III) trifluoromethanesulfonate | 63295-48-7 | 2A-9045889 |
| Ursodeoxycholic-24-13C acid | 63296-46-8 | 2A-9045890 |
| Potassium 3-(phenylsulfonyl)benzenesulfonate | 63316-43-8 | 2A-9045891 |
| Aspoxicillin | 63358-49-6 | 2A-9045897 |
| Metamizole magnesium | 63372-86-1 | 2A-9045899 |
| Polyamide | 63428-84-2 | 2A-9045900 |
| Methyl 4-amino-3-hydroxybenzoate | 63435-16-5 | 2A-9045901 |
| Chlorinated paraffin | 63449-39-8 | 2A-9045903 |
| Benzalkonium chloride | 63449-41-2 | 2A-9045904 |
| Browse all Specialty Chemicals products » | ||
Phone:
630-322-8886
630-322-8887
Email:
Office Locations:
Chicago, IL, USA
San Francisco, CA, USA
EN
SDS