CAS Number54375-47-2
Synonyms2,2'-{[(7-Hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methyl]imino}diacetic acid; 4-Methylumbelliferone-8-methyleneiminodiacetic Acid; Calcein blue; 4-methylumbelliferone-8-methylleneiminodiacetic acid; MFCD00006867; Umbelliferone Complexon; EINECS 259-129-8
Molecular FormulaC15H15NO7
Molecular Weight321.28
Purity98.00%
Density1.5±0.1 g/cm3
Boiling Point636.2±55.0 °C at 760 mmHg
Appearancepowder
EINECS259-129-8
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 54375-47-2 |
| Catalog No. | 2A-9044185 |
| Chinese Name | 钙黄绿素蓝 |
| Synonyms | 2,2'-{[(7-Hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methyl]imino}diacetic acid; 4-Methylumbelliferone-8-methyleneiminodiacetic Acid; Calcein blue; 4-methylumbelliferone-8-methylleneiminodiacetic acid; MFCD00006867; Umbelliferone Complexon; EINECS 259-129-8 |
| Molecular Formula | C15H15NO7 |
| Molecular Weight | 321.28 |
| Purity | 98.00 |
| Density | 1.5±0.1 g/cm3 |
| Boiling Point | 636.2±55.0 °C at 760 mmHg |
| Appearance | powder |
| Storage Condition | Keep the container sealed and stored in a cool, dr |
| Application | Calcein Blue is a short-lasting blue fluorescent dye used to label living cells. |
| EINECS | 259-129-8 |
| HTC | 2934999090 |
| PubChem ID | 24896629 |
| MDL Number | MFCD00006867 |
| SMILES | CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12 |
| InChI | 1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21) |
| InChI Key | RJPSHDMGSVVHFA-UHFFFAOYSA-N |
| NACRES Code | NA.47 |
| UNSPSC | 12171500 |
| Hazard Symbols | transportation |
| MSDS | msds/44185_1_54375_47_2.pdf |
| Bioactivity | Calcein Blue is a short-term, blue-fluorescent dye for labeling live cells. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro Calcein blue AM is only weakly fluorescent (excitation/emission maxima 322/435 nm). Upon cleavage of the AM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for several hours. In addition, its fluorescence intensity increases and its fluorescence spectra shift to longer wavelengths, with excitation/emission maxima of 360/449 nm. The fluorescence of Calcein Blue is known to be quenched in the presence of iron(III); if a stronger chelator removes this ion from the fluorophore, the fluorescence of the fluorophore is regained[1]. A novel fluorimetric assay for dopamine using calcein blue (CB) complexed with Fe2 ion as a chemical sensor is described. The fluorescence arising from calcein blue of the calcein blue-Fe2 complex is quenched by the Fe2 ion. When dopamine is added to a solution of the calcein blue-Fe2 complex, a dopamine-Fe2 complex is formed as the result of a ligand exchange reaction between calcein blue and dopamine which permits the fluorescence from calcein blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the calcein blue-Fe2 complex solution, which permits dopamine to be quantitatively determined[2]. Cell Assay The recommended final working concentrations is usually between 1 and 10 μM to minimize potential artifacts. Generally, 15 minutes to 1 hour is sufficient for cellular uptake and processing of the dyes. A stock concentration of 5 mM 4-methylumbelliferone-8- methyliminodiacetic acid, commonly known as calcein blue is prepared in 0.1 M potassium hydroxide, and the pH is neutralized using 0.1 N hydrochloric acid. The calcein blue stock prepared is diluted to 200 μM (working stock) in DPBS and stored for further use. A concentration of 10 μM calcein blue is prepared from the working stock by diluting in DPBS. From this, 200 μL is added to a 96-well plate and the excitation/emission (Ex./Em.) maximum is determined using the micro-plate reader[1]. References [1]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49. [2]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43. Chemical & Physical Properties Density 1.5±0.1 g/cm3 Boiling Point 636.2±55.0 °C at 760 mmHg Molecular Formula C15H15NO7 Molecular Weight 321.282 Flash Point 338.5±31.5 °C Exact Mass 321.084839 PSA 128.28000 LogP 1.65 Vapour Pressure 0.0±2.0 mmHg at 25°C Index of Refraction 1.642 InChIKey RJPSHDMGSVVHFA-UHFFFAOYSA-N SMILES Cc1cc(=O)oc2c(CN(CC(=O)O)CC(=O)O)c(O)ccc12 Storage condition -20℃ |
| Use of | Calcein Blue is a short-term, blue-fluorescent dye for labeling live cells. Properties Articles20 Name 2-[carboxymethyl-[(7-hydroxy-4-methyl-2-oxochromen-8-yl)methyl]amino]acetic acid Synonym More Synonyms Calcein Blue Biological Activity Description Calcein Blue is a short-term, blue-fluorescent dye for labeling live cells. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others References [1]. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49. [2]. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43. Chemical & Physical Properties Molecular Formula C15H15NO7 Exact Mass 321.084839 PSA 128.28000 Index of Refraction 1.642 InChIKey RJPSHDMGSVVHFA-UHFFFAOYSA-N Storage condition -20℃ |
| Tax Rebate | 13.0% |
| Supervision | None. MFN tariff: 6.5%. Ordinary tariff: 20.0% |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 Names specified by the United Nations (UN) European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Dangerous Regulations: No International Maritime Transport Dangerous Regulations Maritime Pollutants: No International Air Transport Risk Regulations: No 14.6 Special reminder to users No data available |
| Related Products in Biotechnology & Diagnostics | ||
|---|---|---|
| Boc-L-phenylalanine methyl ester | 51987-73-6 | 2A-9043641 |
| 3'-Amino-2',3'-dideoxythymidine | 52450-18-7 | 2A-9043764 |
| Fmoc-S-benzyl-L-cysteine | 53298-33-2 | 2A-9043953 |
| Boc-L-Norvaline | 53308-95-5 | 2A-9043955 |
| 1-Boc-4-methylpiperazine | 53788-49-1 | 2A-9044058 |
| Cytidine-5'-diphosphate disodium salt | 54394-90-0 | 2A-9044193 |
| N-Boc-D-Prolinamide | 54503-10-5 | 2A-9044214 |
| Boc-O-benzyl-trans-4-hydroxy-L-proline | 54631-81-1 | 2A-9044245 |
| Inosine-5'-diphosphoric acid disodium salt | 54735-61-4 | 2A-9044265 |
| N-Boc-L-threonine | 55674-67-4 | 2A-9044448 |
| Browse all Biotechnology & Diagnostics products » | ||
Phone:
630-322-8886
630-322-8887
Email:
Office Locations:
Chicago, IL, USA
San Francisco, CA, USA
EN
SDS