CAS Number23491-52-3
SynonymsbisBenzimide H 33342 trihydrochloride; 2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, trihydrochloride; 2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole; 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride; 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole; 2,6'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-, hydrochloride (1:3); MFCD00166996; BISBENZIMIDE ETHOXIDE TRIHYDROCHLORIDE; 2,5'-bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, hydrochloride (1:3); Hoe 33342; 2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride; EINECS 245-691-1; Hoechst 33342
Molecular FormulaC27H28N6O
Molecular Weight452.56
Purity98.00%
Melting Point268ºC
AppearanceYellow-green powder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 23491-52-3 |
| Catalog No. | 2A-9039266 |
| Chinese Name | 赫斯特荧光染料33342 |
| Synonyms | bisBenzimide H 33342 trihydrochloride; 2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, trihydrochloride; 2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole; 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride; 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole; 2,6'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-, hydrochloride (1:3); MFCD00166996; BISBENZIMIDE ETHOXIDE TRIHYDROCHLORIDE; 2,5'-bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, hydrochloride (1:3); Hoe 33342; 2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride; EINECS 245-691-1; Hoechst 33342 |
| Molecular Formula | C27H28N6O |
| Molecular Weight | 452.56 |
| Purity | 98.00 |
| Melting Point | 268ºC |
| Appearance | Yellow-green powder |
| Storage Condition | 2-8℃ |
| Application | Hoechst dye is a blue fluorescent dye for DNA, and Hoechst 33342 is one of them. |
| HTC | 2933990090 |
| MDL Number | C27H28N6O |
| SMILES | CCOc1ccc(-c2nc3ccc(-c4nc5ccc(N6CCN(C)CC6)cc5[nH]4)cc3[nH]2)cc1 |
| InChI | InChI=1S/C27H28N6O/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31) |
| InChI Key | PRDFBSVERLRRMY-UHFFFAOYSA-N |
| Hazard Symbols | transportation |
| MSDS | msds/39266_1_23491_52_3.pdf |
| Bioactivity | Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. Related Catalog Signaling Pathways >> Autophagy >> Autophagy Dye Reagents Research Areas >> Others Target Dye reagent[1] DNA Stain[1] In Vitro Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1]. Cell Assay Labeling Nuclear DNA with Hoechst 33342[1]Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+.Step 5, Mount the coverslips.Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1]. References [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557. Chemical & Physical Properties Melting Point 268ºC Molecular Formula C27H28N6O Molecular Weight 452.55 PSA 100.76000 LogP 7.13970 InChIKey PRDFBSVERLRRMY-UHFFFAOYSA-N SMILES CCOc1ccc(-c2nc3ccc(-c4nc5ccc(N6CCN(C)CC6)cc5[nH]4)cc3[nH]2)cc1 Storage condition 2-8°C |
| Use of | Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. Properties Articles72 Name 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Synonym More Synonyms Hoechst 33342 Biological Activity Description Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. Related Catalog Signaling Pathways >> Autophagy >> Autophagy Dye Reagents Research Areas >> Others Dye reagent[1] DNA Stain[1] References Chemical & Physical Properties Molecular Formula C27H28N6O PSA 100.76000 LogP 7.13970 InChIKey PRDFBSVERLRRMY-UHFFFAOYSA-N |
| Tax Rebate | 13.0% |
| Supervision | None. MFN tariff: 6.5%. Ordinary tariff: 20.0% |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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