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Cresyl violet acetate structure, CAS 10510-54-0

Cresyl violet acetate

CAS Number10510-54-0

Synonyms5H-benzo[a]phenoxazin-9-amine, 5-imino-, acetate (1:1); Cresyl Violet acetate; MFCD00013151; CRESYL ECHT VIOLETT; Cresyl Violet acetate,certified; Cresyl Violet acetate,certified,pure; CRESYL VIOLET; CRESYL ECHT VIOLET; 5H-Benzo[a]phenoxazin-5-iminium, 9-amino- acetate (1:1); CRESYL VIOLETT; Cresyl Violet acetate,pure,certified; EINECS 234-043-3; 9-Amino-5H-benzo[a]phenoxazin-5-iminium acetate

Molecular FormulaC18H15N3O3

Molecular Weight321.33

Purity98.00%

Density

Boiling Point468.3ºC

Melting Point140-143 °C (lit.)

Flash Point

Appearancepowder

EINECS234-043-3

MSDSChineseEnglish

Symboltransportation

Signal WordWarning

Cat. No.: 2A-9034919 Purity: 98.00%
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Quality Control of [ 10510-54-0 ] Purity: 98.00% SDS Specification MoL

COA NMR CNMR HPLC LC-MS
Chemical & Physical Properties
CAS Number10510-54-0
Catalog No.2A-9034919
Chinese Name焦油紫
Synonyms5H-benzo[a]phenoxazin-9-amine, 5-imino-, acetate (1:1); Cresyl Violet acetate; MFCD00013151; CRESYL ECHT VIOLETT; Cresyl Violet acetate,certified; Cresyl Violet acetate,certified,pure; CRESYL VIOLET; CRESYL ECHT VIOLET; 5H-Benzo[a]phenoxazin-5-iminium, 9-amino- acetate (1:1); CRESYL VIOLETT; Cresyl Violet acetate,pure,certified; EINECS 234-043-3; 9-Amino-5H-benzo[a]phenoxazin-5-iminium acetate
Molecular FormulaC18H15N3O3
Molecular Weight321.33
Purity98.00
Boiling Point468.3ºC
Melting Point140-143 °C (lit.)
Appearancepowder
Storage ConditionSeal in a cool, dry place.
ApplicationCresyl violet acetate is a red fluorescent stain that can be used to stain neurons.
EINECS234-043-3
PubChem ID24892749
MDL NumberMFCD00013151
SMILESCC(O)=O.Nc1cc2OC3=CC(=N)C=CC3=Nc2c4ccccc14
InChI1S/C16H11N3O.C2H4O2/c17-9-5-6-13-14(7-9)20-15-8-12(18)10-3-1-2-4-11(10)16(15)19-13;1-2(3)4/h1-8,17H,18H2;1H3,(H,3,4)
InChI KeyORIKHDNZZCTIAD-UHFFFAOYSA-N
NACRES CodeNA.47
UNSPSC12171500
Hazard Symbolstransportation
MSDSmsds/34919_1_10510_54_0.pdf
BioactivityCresyl violet acetate is a red fluorescent stain, which can be used to stain neurons. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Neurological Disease In Vitro The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in Cresyl violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in Cresyl violet acetate staining and PV immunostaining sections, respectively[1]. Cell Assay Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl violet acetate and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl violet acetate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl violet acetate staining sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1]. References [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6. Chemical & Physical Properties Boiling Point 468.3ºC Melting Point 140-143 °C(lit.) Molecular Formula C18H15N3O3 Molecular Weight 321.330 Flash Point 237ºC Exact Mass 321.111328 PSA 113.20000 LogP 3.91930 Vapour Pressure 6.07E-09mmHg at 25°C InChIKey ORIKHDNZZCTIAD-UHFFFAOYSA-N SMILES CC(=O)O.N=c1ccc2nc3c(cc(N)c4ccccc43)oc-2c1 Stability Stable. Incompatible with strong oxidizing agents.
Use ofCresyl violet acetate is a red fluorescent stain, which can be used to stain neurons. Properties Articles35 Name cresyl violet acetate Synonym More Synonyms Cresyl Violet acetate Biological Activity Description Cresyl violet acetate is a red fluorescent stain, which can be used to stain neurons. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Neurological Disease References [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6. Chemical & Physical Properties Molecular Formula C18H15N3O3 Exact Mass 321.111328 PSA 113.20000 LogP 3.91930 InChIKey ORIKHDNZZCTIAD-UHFFFAOYSA-N Stability Stable. Incompatible with strong oxidizing agents.
Transport InfoShipping Name: UN
MSDS Transport InfoModule 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available
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