CAS Number3520-43-2
Synonyms5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide; 5,5'6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide; 1,1',3,3'-tetraethyl-5,5'6,6'-tetrachlorobenzimidazolocarbocyanine iodide; 1,3,1',3'-Tetraethyl-5,6,5',6'-tetrachloro-2,2'-imidacarbocyanine iodide; JC-1; 5,5',6,6'-TETRACHLORO-1,1',3,3'-*TETRAETHYBENZIMIDA
Molecular FormulaC25H27Cl4IN4
Molecular Weight652.23
Purity≥97%
Appearancepowder or solid (Crystals)
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 3520-43-2 |
| Catalog No. | 2A-9029900 |
| Chinese Name | CBIC2 |
| Synonyms | 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide; 5,5'6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine iodide; 1,1',3,3'-tetraethyl-5,5'6,6'-tetrachlorobenzimidazolocarbocyanine iodide; 1,3,1',3'-Tetraethyl-5,6,5',6'-tetrachloro-2,2'-imidacarbocyanine iodide; JC-1; 5,5',6,6'-TETRACHLORO-1,1',3,3'-*TETRAETHYBENZIMIDA |
| Molecular Formula | C25H27Cl4IN4 |
| Molecular Weight | 652.23 |
| Purity | ≥97 |
| Appearance | powder or solid (Crystals) |
| Storage Condition | -20℃ |
| Application | JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. |
| PubChem ID | 24900193 |
| MDL Number | MFCD00188475 |
| SMILES | [I-].CCN1C(=C\C=C\c2n(CC)c3cc(Cl)c(Cl)cc3[n+]2CC)/N(CC)c4cc(Cl)c(Cl)cc14 |
| InChI | 1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1 |
| InChI Key | FYNNIUVBDKICAX-UHFFFAOYSA-M |
| NACRES Code | NA.47 |
| UNSPSC | 12171500 |
| Hazard Symbols | transportation |
| MSDS | msds/29900_1_3520_43_2.pdf |
| Bioactivity | JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro JC-1 (2.5 μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers[1]. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4]. References [1]. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430. [2]. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462(5): 693-708. [3]. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323. [4]. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82. Chemical & Physical Properties Molecular Formula C25H27Cl4IN4 Molecular Weight 652.225 Exact Mass 650.003418 PSA 18.67000 LogP 4.15720 InChIKey FYNNIUVBDKICAX-UHFFFAOYSA-M SMILES CCN1C(=CC=Cc2n(CC)c3cc(Cl)c(Cl)cc3[n+]2CC)N(CC)c2cc(Cl)c(Cl)cc21.[I-] Storage condition -20℃ |
| Use of | JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. Properties Articles366 Name 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide Synonym More Synonyms JC-1 Biological Activity Description JC-1 is a fluorescent lipophilic carbocyanine dye used to measure mitochondrial membrane potential. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others References [1]. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430. [2]. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462(5): 693-708. [3]. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323. [4]. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82. Chemical & Physical Properties Exact Mass 650.003418 PSA 18.67000 LogP 4.15720 InChIKey FYNNIUVBDKICAX-UHFFFAOYSA-M Storage condition -20℃ |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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