CAS Number3326-32-7
Synonyms5-FITC; Isothiocyanatofluorescein; 5-Isothionatofluorescein; FITC Fluorescein 5-isothiocyanate; EINECS 248-207-7; Fluorescein Isothiosyanate; fluorescein isothiocyanate isomer 1; fluorescein 6-isothiocyanate; FITC ISOMER; Fluorescein 5-Isothiocyanate (isomer I); 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one; 5-FITC (isomer I); 5-Isothiocyanatofluorescein (isomer I); FITC; 5-Fluorescein Isothiocyanate; fluorescein 5-isothiocyanate; FITC,Fluorescein 5-isothiocyanate; FITC isomer I; Fluorescein isothiocyanate,isomer 1; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid; Fluorescein Isothiocyanate (isomer I); Fluorescein isothiocyanate isomer I; FITC-I; Fluorescein 5(6)-isothiocyanate; Fluorescein-5-isothiocyanate; MFCD00005063; Fluorescein Isothiocy
Molecular FormulaC21H11NO5S
Molecular Weight389.38
Purity98.00%
Density1.5±0.1 g/cm3
Boiling Point708.6±60.0 °C at 760 mmHg
Melting Point>360 °C(lit.)
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 3326-32-7 |
| Catalog No. | 2A-9029677 |
| Chinese Name | 异硫氰酸荧光素酯 |
| Synonyms | 5-FITC; Isothiocyanatofluorescein; 5-Isothionatofluorescein; FITC Fluorescein 5-isothiocyanate; EINECS 248-207-7; Fluorescein Isothiosyanate; fluorescein isothiocyanate isomer 1; fluorescein 6-isothiocyanate; FITC ISOMER; Fluorescein 5-Isothiocyanate (isomer I); 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one; 5-FITC (isomer I); 5-Isothiocyanatofluorescein (isomer I); FITC; 5-Fluorescein Isothiocyanate; fluorescein 5-isothiocyanate; FITC,Fluorescein 5-isothiocyanate; FITC isomer I; Fluorescein isothiocyanate,isomer 1; 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid; Fluorescein Isothiocyanate (isomer I); Fluorescein isothiocyanate isomer I; FITC-I; Fluorescein 5(6)-isothiocyanate; Fluorescein-5-isothiocyanate; MFCD00005063; Fluorescein Isothiocy |
| Molecular Formula | C21H11NO5S |
| Molecular Weight | 389.38 |
| Purity | 98.00 |
| Density | 1.5±0.1 g/cm3 |
| Boiling Point | 708.6±60.0 °C at 760 mmHg |
| Melting Point | >360 °C(lit.) |
| Appearance | powder |
| Water Solubility | practically insoluble |
| Storage Condition | Store at room temperature, away from light, in a v |
| Application | FITC is a derivative of fluorescein and is used to label amines. |
| PubChem ID | 24894860 |
| SMILES | O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21 |
| InChI | InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| InChI Key | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| Hazard Symbols | transportation |
| Bioactivity | FITC is a derivative of fluorescein for the labeling of amines. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2]. Cell Assay The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate. References [1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5. [2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71 Chemical & Physical Properties Density 1.5±0.1 g/cm3 Boiling Point 708.6±60.0 °C at 760 mmHg Melting Point >360 °C(lit.) Molecular Formula C21H11NO5S Molecular Weight 389.381 Flash Point 382.4±32.9 °C Exact Mass 389.035797 PSA 120.44000 LogP 4.00 Vapour Pressure 0.0±2.3 mmHg at 25°C Index of Refraction 1.754 InChIKey MHMNJMPURVTYEJ-UHFFFAOYSA-N SMILES O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N=C=S)cc21 Storage condition 2-8°C Stability Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids. Water Solubility practically insoluble |
| Use of | FITC is a derivative of fluorescein for the labeling of amines. Properties Articles2410 Name fluorescein 5-isothiocyanate Synonym More Synonyms Fluorescein isothiocyanate Biological Activity Description FITC is a derivative of fluorescein for the labeling of amines. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2]. References [1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5. [2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71 Chemical & Physical Properties Molecular Formula C21H11NO5S Exact Mass 389.035797 PSA 120.44000 Index of Refraction 1.754 InChIKey MHMNJMPURVTYEJ-UHFFFAOYSA-N Stability Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids. Water Solubility practically insoluble |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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