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Xylene Cyanole FF structure, CAS 2650-17-1

Xylene Cyanole FF

CAS Number2650-17-1

SynonymsXylene Cyanol FF; Acid Blue 147; XYLENE CYANOL; CYANOL BLUE; EINECS 224-728-5; Xylene Cyanole F; CYANOL; Blue 147; Acid blue 147,Cyanol FF,XC; MFCD00040657; CYANOL FF

Molecular FormulaC25H27N2NaO6S2

Molecular Weight538.61

Purity98.00%

Density

Boiling Point

Melting Point295°C

Flash Point

AppearanceDark green or blue-gray crystals

EINECS220-167-5

MSDSChineseEnglish

Symboltransportation

Signal WordWarning

Cat. No.: 2A-9028865 Purity: 98.00%
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Quality Control of [ 2650-17-1 ] Purity: 98.00% SDS Specification MoL

COA NMR CNMR HPLC LC-MS
Chemical & Physical Properties
CAS Number2650-17-1
Catalog No.2A-9028865
Chinese Name二甲苯青FF
SynonymsXylene Cyanol FF; Acid Blue 147; XYLENE CYANOL; CYANOL BLUE; EINECS 224-728-5; Xylene Cyanole F; CYANOL; Blue 147; Acid blue 147,Cyanol FF,XC; MFCD00040657; CYANOL FF
Molecular FormulaC25H27N2NaO6S2
Molecular Weight538.61
Purity98.00
Melting Point295°C
AppearanceDark green or blue-gray crystals
Water SolubilityWater solubility: completely dissolved
Storage ConditionSealed in a cool, dry environment
ApplicationXylene Blue FF is an acidic triphenylmethane dye. Xylene Blue FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. With the ad
EINECS220-167-5
HTC32041900
PubChem ID24860353
SMILESCCN=C1C=CC(=C(c2ccc(NCC)c(C)c2)c2ccc(S(=O)(=O)[O-])cc2S(=O)(=O)O)C=C1C.[Na+]
InChIInChI=1S/C25H28N2O6S2.Na/c1-4-26-23-9-5-19(13-17(23)2)11-12-27-24-10-6-20(14-18(24)3)15-21-7-8-22(34(28,29)30)16-25(21)35(31,32)33;/h5-10,13-16,26H,4,11-12H2,1-3H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b20-15+,27-24?;
InChI KeyVVLFAAMTGMGYBS-LZMHHRBESA-M
Hazard Symbolstransportation
Risk CodesS24/25
BioactivityXylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. in the solution to be tested[1][2]. Related Catalog Research Areas >> Others In Vitro Xylene Cyanol FF can trace DNA in different polyacrylamide gel electrophoresis. The operation is as follows [1]: 1. Used for denaturing gel. (1) Prepare gel: 10% acrylamide (19:1, acrylamide:bisacrylamide), and 8.3 M urea. The usage condition is 55℃. (2) Prepare electrophoresis buffer: 89 mM Tris, HCl, pH 8.0, 89 mM boric acid, 2 mM EDTA (TBE). (3) Prepare loading buffer: 10 mM NaOH, 1 mM EDTA, and 0.1% Xylene Cyanol FF. (4) The gel was run on an IBI model STS 45 electrophoresis unit at 70 W (50 V/cm, constant power) or on a Hoefer SE 600 electrophoresis unit at 60 °C (31 V/cm, constant voltage). (5) Place the gel on Whatman 3MM paper to dry and expose to X-ray film. Exposure time is up to 15 hours. 2. Used for denaturing gels. (1) Prepare gel: containing 8% acrylamide (19:1, acrylamide:bisacrylamide). (2) Prepare DNA suspension: containing 40 mM TrisβHCl, pH 8.0, 20 mM acetic acid, 2 mM EDTA, and 12.5 mM magnesium acetate (TAEMg). (3) Boil the DNA suspension and cool slowly to 16 °C. (4) Prepare staining solution: containing TAEMg, 50% glycerol, 0.02% bromophenol blue and 0.02% Xylene Cyanol FF. Bring the sample to a final volume of 10 µL with this staining solution. (5) Gels were run on a Hoefer SE-600 gel electrophoresis device at 11 V/cm at 16 °C and exposed to X-ray film for up to 15 h or stained with Stainsall (HY-D0987) dye. References [1]. Li X, et al. Antiparallel DNA double crossover molecules as components for nanoconstruction. Journal of the American Chemical Society, 1996, 118(26): 6131-6140. [2]. Cai L, et al. Society, 2011, 22: 1987-1992. Chemical & Physical Properties Melting Point 295°C Molecular Formula C25H27N2NaO6S2 Molecular Weight 538.611 Exact Mass 538.120850 PSA 152.72000 LogP 6.59110 InChIKey VVLFAAMTGMGYBS-LZMHHRBESA-M SMILES CCN=C1C=CC(=C(c2ccc(NCC)c(C)c2)c2ccc(S(=O)(=O)[O-])cc2S(=O)(=O)O)C=C1C.[Na+] Storage condition Store at RT.
Use ofXylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested[1][2]. Properties Articles26 Name Xylene Cyanole FF Synonym More Synonyms Xylene Cyanole F Biological Activity Description Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested[1][2]. Related Catalog Research Areas >> Others References [1]. Li X, et al. Antiparallel DNA double crossover molecules as components for nanoconstruction. Journal of the American Chemical Society, 1996, 118(26): 6131-6140. [2]. Cai L, et al. Determination of iron and aluminum based on the catalytic effect on the reaction of xylene cyanol FF with hydrogen peroxide and potassium periodate[J]. Journal of the Brazilian Chemical Society, 2011, 22: 1987-1992. Chemical & Physical Properties Molecular Formula C25H27N2NaO6S2 Exact Mass 538.120850 PSA 152.72000 LogP 6.59110 InChIKey VVLFAAMTGMGYBS-LZMHHRBESA-M Storage condition Store at RT.
Transport InfoShipping Name: UN
MSDS Transport InfoModule 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available
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