CAS Number2321-07-5
SynonymsD and C Yellow No. 7; D&C Yellow No. 7; D+C Yellow No. 7; Fluorescein (free acid); 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 3',6'-Fluorandiol; Fluorecein; Eosin; YELLOW 7; 3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; KI201; MFCD00005050; Acid Yellow 73; 3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one; Fluorescite; EINECS 219-031-8; Uranine Yellow; C.I. Acid Yellow 73; angiofluor; Soluble fluorescein; D & C Yellow no. 7; Fluor-I-Strip; D & C Yellow no. 8; Fluorescein; Ful-Glo; Funduscein
Molecular FormulaC20H12O5
Molecular Weight332.31
Purity98.00%
Density1.6±0.1 g/cm3
Boiling Point620.8±55.0 °C at 760 mmHg
Melting Point320 °C(lit.)
AppearanceOrange-red crystalline powder
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 2321-07-5 |
| Catalog No. | 2A-9028315 |
| Chinese Name | 荧光素 |
| Synonyms | D and C Yellow No. 7; D&C Yellow No. 7; D+C Yellow No. 7; Fluorescein (free acid); 3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 3',6'-Fluorandiol; Fluorecein; Eosin; YELLOW 7; 3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; KI201; MFCD00005050; Acid Yellow 73; 3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one; Fluorescite; EINECS 219-031-8; Uranine Yellow; C.I. Acid Yellow 73; angiofluor; Soluble fluorescein; D & C Yellow no. 7; Fluor-I-Strip; D & C Yellow no. 8; Fluorescein; Ful-Glo; Funduscein |
| Molecular Formula | C20H12O5 |
| Molecular Weight | 332.31 |
| Purity | 98.00 |
| Density | 1.6±0.1 g/cm3 |
| Boiling Point | 620.8±55.0 °C at 760 mmHg |
| Melting Point | 320 °C(lit.) |
| Appearance | Orange-red crystalline powder |
| Water Solubility | insoluble |
| Storage Condition | Keep container tightly sealed in a tight-fitting c |
| Packaging | I; II; III |
| Application | Fluorescein is a synthetic fluorescent tracer. |
| HTC | 3204200000 |
| PubChem ID | 329749868 |
| SMILES | O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccccc21 |
| InChI | InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H |
| InChI Key | GNBHRKFJIUUOQI-UHFFFAOYSA-N |
| Bioactivity | Fluorescein is a synthetic fluorescent tracer. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent[1]. References [1]. R Sjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Chemical & Physical Properties Density 1.6±0.1 g/cm3 Boiling Point 620.8±55.0 °C at 760 mmHg Melting Point 320 °C(lit.) Molecular Formula C20H12O5 Molecular Weight 332.306 Flash Point 232.6±25.0 °C Exact Mass 332.068481 PSA 87.74000 LogP 2.98 Vapour Pressure 0.0±1.9 mmHg at 25°C Index of Refraction 1.792 InChIKey GNBHRKFJIUUOQI-UHFFFAOYSA-N SMILES O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccccc21 Storage condition −20°C Water Solubility insoluble |
| Use of | Fluorescein is a synthetic fluorescent tracer. Properties Articles1822 Name fluorescein Synonym More Synonyms Fluorescein Biological Activity Description Fluorescein is a synthetic fluorescent tracer. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent[1]. References [1]. R Sjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Chemical & Physical Properties Molecular Formula C20H12O5 Exact Mass 332.068481 PSA 87.74000 Index of Refraction 1.792 InChIKey GNBHRKFJIUUOQI-UHFFFAOYSA-N Water Solubility insoluble |
| Tax Rebate | 13.0% |
| Supervision | None. MFN tariff: 6.5%. Ordinary tariff: 20.0% |
| Transport Info | Product name: Purity: 0.0% |
| MSDS Transport Info | Module 14. Shipping information United Nations classification: inconsistent with United Nations classification standards UN number: not specified |
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