CAS Number1461-15-0
SynonymsFluorescein-bis(methyliminodiacetic acid); Oftasceinum; Fluorexon; EINECS 215-957-1; Fluorescein complexon; Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein; Oftasceina; calcein; Oftasceine; MFCD00005049; oftasceinum [INN_la]
Molecular FormulaC30H26O13N2
Molecular Weight622.54
Purity98.00%
Density1.7±0.1 g/cm3
Boiling Point952.7±65.0 °C at 760 mmHg
Melting Point200 °C
Appearanceorange powder
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 1461-15-0 |
| Catalog No. | 2A-9026853 |
| Chinese Name | 钙黄绿素 |
| Synonyms | Fluorescein-bis(methyliminodiacetic acid); Oftasceinum; Fluorexon; EINECS 215-957-1; Fluorescein complexon; Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein; Oftasceina; calcein; Oftasceine; MFCD00005049; oftasceinum [INN_la] |
| Molecular Formula | C30H26O13N2 |
| Molecular Weight | 622.54 |
| Purity | 98.00 |
| Density | 1.7±0.1 g/cm3 |
| Boiling Point | 952.7±65.0 °C at 760 mmHg |
| Melting Point | 200 °C |
| Appearance | orange powder |
| Water Solubility | insoluble |
| Storage Condition | room temperature |
| Application | Calcein is a fluorescent dye and self-extinguishing probe that can be used as an indicator of lipid vesicle leakage, and can also be used as a complex indicator for EDTA calcium ion titration and fluo |
| HTC | 32041900 |
| PubChem ID | 147488 |
| MDL Number | MFCD00005049 |
| SMILES | O=C(O)CN(CC(=O)O)Cc1cc2c(cc1O)Oc1cc(O)c(CN(CC(=O)O)CC(=O)O)cc1C21OC(=O)c2ccccc21 |
| InChI | InChI=1S/C30H26N2O13/c33-21-7-23-19(5-15(21)9-31(11-25(35)36)12-26(37)38)30(18-4-2-1-3-17(18)29(43)45-30)20-6-16(22(34)8-24(20)44-23)10-32(13-27(39)40)14-28(41)42/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42) |
| InChI Key | DEGAKNSWVGKMLS-UHFFFAOYSA-N |
| MSDS | msds/26853_1_1461_15_0.pdf |
| Bioactivity | Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control. Cell Assay Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates. References [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203. Chemical & Physical Properties Density 1.7±0.1 g/cm3 Boiling Point 952.7±65.0 °C at 760 mmHg Melting Point 200 °C Molecular Formula C30H26N2O13 Molecular Weight 622.533 Flash Point 530.0±34.3 °C Exact Mass 622.143494 PSA 231.67000 LogP 1.56 Vapour Pressure 0.0±0.3 mmHg at 25°C Index of Refraction 1.775 InChIKey DEGAKNSWVGKMLS-UHFFFAOYSA-N SMILES O=C(O)CN(CC(=O)O)Cc1cc2c(cc1O)Oc1cc(O)c(CN(CC(=O)O)CC(=O)O)cc1C21OC(=O)c2ccccc21 Stability Stable. Incompatible with strong oxidizing agents. Water Solubility insoluble |
| Use of | Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Properties Name calcein Synonym More Synonyms calcein Biological Activity Description Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others In Vitro Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control. References [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203. Chemical & Physical Properties Molecular Formula C30H26N2O13 Exact Mass 622.143494 PSA 231.67000 Index of Refraction 1.775 InChIKey DEGAKNSWVGKMLS-UHFFFAOYSA-N Stability Stable. Incompatible with strong oxidizing agents. Water Solubility insoluble |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information United Nations classification: inconsistent with United Nations classification standards UN number: not specified |
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