
CAS Number298-83-9
Synonymsnitro blue tetrazolium chloride; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-(3,3'-dimethoxy)-4,4'-bisphenyleneditetrazolium Chloride; Nitrotetrazolium Blue chloride (NBT); 3,3'-(3,3'-Dimethoxybiphenyl-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] dichloride; NBT Vials; NBTMF; Nitroterazoium blue chlorid; nitro blue tetrazolium dichloride; P-NBT; MFCD00150013; NBT; nitrotetrazoliumbt; Nitro Blue Tetrazolium; 3,3'-(3,3'-Dimethoxy-4,4'-biphenyldiyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] dichloride; EINECS 206-067-4; Nitro-Tb; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; Nitro Blue Tetrazolium Chloride monohydrate; DITETRAZOLIUM DYE; Nitrotetrazolium Blue chloride; NBT CHLORIDE; p-Nitro-Blue tetrazolium chloride; NITRO BT
Molecular FormulaC40H30N10O6 · 2Cl
Molecular Weight817.64
Purity≥90.0 (HPLC)%
Density1.5521 (rough estimate)
Melting Point200ºC
Appearancepowder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 298-83-9 |
| Catalog No. | 2A-9021143 |
| Chinese Name | 氯化硝基四氮唑蓝 |
| Synonyms | nitro blue tetrazolium chloride; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-(3,3'-dimethoxy)-4,4'-bisphenyleneditetrazolium Chloride; Nitrotetrazolium Blue chloride (NBT); 3,3'-(3,3'-Dimethoxybiphenyl-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] dichloride; NBT Vials; NBTMF; Nitroterazoium blue chlorid; nitro blue tetrazolium dichloride; P-NBT; MFCD00150013; NBT; nitrotetrazoliumbt; Nitro Blue Tetrazolium; 3,3'-(3,3'-Dimethoxy-4,4'-biphenyldiyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] dichloride; EINECS 206-067-4; Nitro-Tb; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; Nitro Blue Tetrazolium Chloride monohydrate; DITETRAZOLIUM DYE; Nitrotetrazolium Blue chloride; NBT CHLORIDE; p-Nitro-Blue tetrazolium chloride; NITRO BT |
| Molecular Formula | C40H30N10O6 · 2Cl |
| Molecular Weight | 817.64 |
| Purity | ≥90.0 (HPLC) |
| Density | 1.5521 (rough estimate) |
| Melting Point | 200ºC |
| Appearance | powder |
| Water Solubility | methanol: 50 mg/mL, clear, very strongly yellow | |
| Storage Condition | 2-8℃ |
| Application | NBT (Nitro BT; p-Nitrotetrazolium blue) is a dehydrogenase substrate and is used together with the alkaline phosphatase substrate BCIP for western blotting and immunohistochemical staining. |
| HTC | 32041900 |
| PubChem ID | 24897795 |
| MDL Number | MFCD00012159 |
| SMILES | [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8 |
| InChI | 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2 |
| InChI Key | FSVCQIDHPKZJSO-UHFFFAOYSA-L |
| Beilstein/REAXYS | 4115923 |
| NACRES Code | NA.47 |
| UNSPSC | 12171500 |
| Hazard Symbols | Transport |
| MSDS | msds/21143_1_298_83_9.pdf |
| Bioactivity | NBT(Nitro BT;p-Nitrotetrazolium blue) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others Chemical & Physical Properties Density 1.5521 (rough estimate) Melting Point 200ºC Molecular Formula C40H30Cl2N10O6 Molecular Weight 817.636 Exact Mass 816.172668 PSA 179.28000 LogP 1.29040 Index of Refraction 1.7350 (estimate) InChIKey FSVCQIDHPKZJSO-UHFFFAOYSA-L SMILES COc1cc(-c2ccc(-[n+]3nc(-c4ccccc4)nn3-c3ccc([N+](=O)[O-])cc3)c(OC)c2)ccc1-[n+]1nc(-c2ccccc2)nn1-c1ccc([N+](=O)[O-])cc1.[Cl-].[Cl-] Storage condition 2-8°C Water Solubility methanol: 50 mg/mL, clear, very strongly yellow | Soluble in water, ethanol and methanol. |
| Use of | NBT(Nitro BT;p-Nitrotetrazolium blue) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. Properties Articles601 Name nitro blue tetrazolium dichloride Synonym More Synonyms NBT Biological Activity Description NBT(Nitro BT;p-Nitrotetrazolium blue) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. Related Catalog Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others Chemical & Physical Properties Exact Mass 816.172668 PSA 179.28000 LogP 1.29040 Index of Refraction 1.7350 (estimate) InChIKey FSVCQIDHPKZJSO-UHFFFAOYSA-L |
| Transport Info | Product name: Purity: 98.0% |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 Names specified by the United Nations (UN) European Land Transport Dangerous Regulations: No dangerous goods IMDG Code: No dangerous goods International Air Transport Dangerous Regulations: No dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Dangerous Regulations: No International Maritime Transport Dangerous Regulations Maritime Pollutants: No International Air Transport Risk Regulations: No 14.6 Special reminder to users No data available |
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