CAS Number150206-05-6
SynonymsCarboxyfluorescein diacetate succinimidyl ester; 5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester; 5-CFDA N-succinimidyl ester; (2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate; 5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester
Molecular FormulaC29H19NO11
Molecular Weight557.46
Density1.6±0.1 g/cm3
Boiling Point757.9±70.0 °C at 760 mmHg
Melting Point152-154ºC(lit.)
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 150206-05-6 |
| Catalog No. | 2A-2187501 |
| Chinese Name | 5-(((2,5-二氧吡咯烷-1-基)氧基)羰基)-3-羰基-3H-螺-[异苯并呋喃-1,9'-氧杂蒽]-3',6'-二基 二乙酸酯 |
| Synonyms | Carboxyfluorescein diacetate succinimidyl ester; 5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester; 5-CFDA N-succinimidyl ester; (2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate; 5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.46 |
| Density | 1.6±0.1 g/cm3 |
| Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Melting Point | 152-154ºC(lit.) |
| Storage Condition | -20℃, inert gas |
| Application | 5-Carboxyfluorescein diacetate N-succinimide ester is a cell-penetrating dye (Ex=492nm, Em=517nm). 5-Carboxyfluorescein diacetate N-succinimide ester can label cells by covalently binding to intracell |
| PubChem ID | 57653248 |
| MDL Number | MFCD00037466 |
| SMILES | CC(=O)Oc1ccc2c(c1)Oc1cc(OC(C)=O)ccc1C21OC(=O)c2cc(C(=O)ON3C(=O)CCC3=O)ccc21 |
| InChI | InChI=1S/C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3 |
| InChI Key | SRSXVRUMXPCNAJ-UHFFFAOYSA-N |
| NACRES Code | NA.32 |
| UNSPSC | 12352108 |
| Bioactivity | 5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Dilute the stock solution in PBS (to give a 50 μM solution). 2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly. 3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS. 4. Centrifuge cells 5 min at 300 ×g, 20 ℃. 5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS. 6. Centrifuge cells 5 min at 300 × g, 20 ℃, and removing the supernatant. Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled. References [1]. Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9. Chemical & Physical Properties Density 1.6±0.1 g/cm3 Boiling Point 757.9±70.0 °C at 760 mmHg Melting Point 152-154ºC(lit.) Molecular Formula C29H19NO11 Molecular Weight 557.461 Flash Point 412.2±35.7 °C Exact Mass 557.095825 PSA 151.81000 LogP 0.50 Vapour Pressure 0.0±2.6 mmHg at 25°C Index of Refraction 1.701 InChIKey SRSXVRUMXPCNAJ-UHFFFAOYSA-N SMILES CC(=O)Oc1ccc2c(c1)Oc1cc(OC(C)=O)ccc1C21OC(=O)c2cc(C(=O)ON3C(=O)CCC3=O)ccc21 |
| Use of | 5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1]. Properties Articles1002 Name 5-Carboxy-fluorescein diacetate N-succinimidyl ester Synonym More Synonyms Biological Activity Description 5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1]. Related Catalog Research Areas >> Others Signaling Pathways >> Others >> Others References [1]. Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9. Chemical & Physical Properties Molecular Formula C29H19NO11 Exact Mass 557.095825 PSA 151.81000 Index of Refraction 1.701 InChIKey SRSXVRUMXPCNAJ-UHFFFAOYSA-N |
| Transport Info | Product name: 5-(((25-dioxopyrrolidin-1-yl)oxy)carbonyl)-3-oxo-3H-spiro[isobenzofuran-19'-xanthene]-3'6'-diethyldiacetate |
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