CAS Number62796-29-6
SynonymsMFCD00042001; 5-chlorosulfonyl-2-[3-(diethylamino)-6-diethylazaniumylidenexanthen-9-yl]benzenesulfonate; EINECS 263-735-8; Sulforhodamine B acid chloride
Molecular FormulaC27H29ClN2O6S2
Molecular Weight577.11200
Purity98%%
Density1.1576 (rough estimate)
AppearanceDark green crystalline powder
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 62796-29-6 |
| Catalog No. | 2A-1171927 |
| Chinese Name | 丽丝胺碱性蕊香红B磺酰氯 |
| Synonyms | MFCD00042001; 5-chlorosulfonyl-2-[3-(diethylamino)-6-diethylazaniumylidenexanthen-9-yl]benzenesulfonate; EINECS 263-735-8; Sulforhodamine B acid chloride |
| Molecular Formula | C27H29ClN2O6S2 |
| Molecular Weight | 577.11200 |
| Purity | 98% |
| Density | 1.1576 (rough estimate) |
| Appearance | Dark green crystalline powder |
| Storage Condition | Sealed in a cool, dry environment at -20 ºC |
| Application | Sulforhodamine B acid chloride is a fluorescent protein marker that forms stable conjugates. |
| HTC | 2921199090 |
| PubChem ID | 3156986 |
| SMILES | CCN(CC)c1ccc2c(-c3ccc(S(=O)(=O)Cl)cc3S(=O)(=O)[O-])c3ccc(=[N+](CC)CC)cc-3oc2c1 |
| InChI | InChI=1S/C27H29ClN2O6S2/c1-5-29(6-2)18-9-12-21-24(15-18)36-25-16-19(30(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(28,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3 |
| InChI Key | YERWMQJEYUIJBO-UHFFFAOYSA-N |
| Hazard Symbols | transportation |
| MSDS | msds/172147_2_62796_29_6.pdf |
| Bioactivity | Sulforhodamine B acid chloride is a fluorescent protein label forming stable conjugates. Related Catalog Signaling Pathways >> Others >> Others Research Areas >> Others Dye Reagents In Vitro Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1]. Cell Assay Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1]. References [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. Chemical & Physical Properties Density 1.1576 (rough estimate) Molecular Formula C27H29ClN2O6S2 Molecular Weight 577.11200 Exact Mass 576.11600 PSA 127.49000 LogP 6.85600 Index of Refraction 1.6100 (estimate) InChIKey YERWMQJEYUIJBO-UHFFFAOYSA-N SMILES CCN(CC)c1ccc2c(-c3ccc(S(=O)(=O)Cl)cc3S(=O)(=O)[O-])c3ccc(=[N+](CC)CC)cc-3oc2c1 Storage condition −20°C |
| Use of | Sulforhodamine B acid chloride is a fluorescent protein label forming stable conjugates. Properties Name lissamine rhodamine b sulfonyl chloride Synonym More Synonyms lissamine rhodamine b sulfonyl chloride Biological Activity Description Sulforhodamine B acid chloride is a fluorescent protein label forming stable conjugates. Related Catalog Signaling Pathways >> Others >> Others Research Areas >> Others Dye Reagents In Vitro Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1]. References [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. Chemical & Physical Properties Exact Mass 576.11600 PSA 127.49000 LogP 6.85600 Index of Refraction 1.6100 (estimate) InChIKey YERWMQJEYUIJBO-UHFFFAOYSA-N |
| Tax Rebate | 9.0% |
| Supervision | none |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
| Related Products in Pigments & Additives | ||
|---|---|---|
| Acid Yellow 220 | 71603-79-7 | 2A-1169805 |
| C.I. Acid Yellow 232 | 134687-50-6 | 2A-1169806 |
| Acid red 414 | 152287-09-7 | 2A-1169819 |
| Benzenesulfonic acid, 4-amino-, diazotized, coupled with Dyer's mulberry extract | 71949-32-1 | 2A-1169836 |
| SOLVENT RED 195 | 164251-88-1 | 2A-1170578 |
| Solvent Red 30 | 6226-87-5 | 2A-1172243 |
| Disperse Yellow 54 | 12223-85-7 | 2A-1172520 |
| Basic Blue 57 | 12221-31-7 | 2A-1174526 |
| LEUCOMALACHITE GREEN | 129-73-7 | 2A-2180469 |
| Phthalocyanine Blue 15:3 | 2A-1182413 | 2A-1182413 |
| Browse all Pigments & Additives products » | ||
Phone:
630-322-8886
630-322-8887
Email:
Office Locations:
Chicago, IL, USA
San Francisco, CA, USA
EN
SDS