CAS Number1226056-71-8
SynonymsThiazovivin; N-Benzyl-2-(pyrimidin-4-ylamino)-1,3-thiazole-4-carboxamide; N-Benzyl-2-(4-pyrimidinylamino)-1,3-thiazole-4-carboxamide
Molecular FormulaC15H13N5OS
Molecular Weight311.362
Purity98%%
Density1.4±0.1 g/cm3
Appearance, white to beige to brown
Symbol
Signal WordWarning
| Chemical & Physical Properties | |
|---|---|
| CAS Number | 1226056-71-8 |
| Catalog No. | 2A-1170125 |
| Chinese Name | Thiazovivin |
| Synonyms | Thiazovivin; N-Benzyl-2-(pyrimidin-4-ylamino)-1,3-thiazole-4-carboxamide; N-Benzyl-2-(4-pyrimidinylamino)-1,3-thiazole-4-carboxamide |
| Molecular Formula | C15H13N5OS |
| Molecular Weight | 311.362 |
| Purity | 98% |
| Density | 1.4±0.1 g/cm3 |
| Appearance | , white to beige to brown |
| Water Solubility | DMSO: soluble20mg/mL, clear |
| Storage Condition | Store at -20°C |
| Application | Thiazovivin is a potent ROCK inhibitor with protective effects on human embryonic stem cells. |
| HTC | 2934100090 |
| PubChem ID | 96063683 |
| InChI | InChI=1S/C15H13N5OS/c21-14(17-8-11-4-2-1-3-5-11)12-9-22-15(19-12)20-13-6-7-16-10-18-13/h1-7,9-10H,8H2,(H,17,21)(H,16,18,19,20) |
| InChI Key | DOBKQCZBPPCLEG-UHFFFAOYSA-N |
| Hazard Symbols | transportation |
| MSDS | msds/170263_2_1226056_71_8.pdf |
| Bioactivity | Thiazovivin is a potent ROCK inhibitor, which can protect human embryonic stem cells. Related Catalog Signaling Pathways >> Cell Cycle/DNA Damage >> ROCK Signaling Pathways >> Stem Cell/Wnt >> ROCK Signaling Pathways >> TGF-beta/Smad >> ROCK Research Areas >> Cancer Target ROCK In Vitro Thiazovivin is a ROCK inhibitor. Thiazovivin (2 μM) inhibits ROCK activity and protects human embryonic stem cells (hESCs). Thiazovivin significantly increases the survival of hESCs after dissociation while maintaining pluripotency. Thiazovivin enhances cell-ECM adhesion-mediated integrin signaling. Thiazovivin also stabilizes E-cadherin after cell dissociation to protect hESCs from death under ECM-free conditions[1]. Thiazovivin increases cellular attachment of embryo-derived stem-like cells (eSLCs) of cattle and formation of primary colonies on the feeder layer. Thiazovivin reinforces putative colony outgrowth and supports the expansion of eSLC cultures during the subculture for passaging. Furthermore, Thiazovivin causes greater expression of ectodermal lineage-specific genes in eSLCs of cattle[2]. Cell Assay For cell proliferation assays, embryo-derived stem-like cells (eSLCs) are newly passaged and cultured in 3i system for 48 h on the feeder-free condition to prevent contamination of the BrdU-positive feeder cells. Cells are fixed with 4% paraformaldehyde in PBS (pH 7.4) at 37°C for 2 h, acid-treated with 2 N HCl in PBS for 30 min at 45°C, equilibrated with 0.1 M borate buffer (pH 8.5), and finally incubated with blocking buffer (20% Calf serum; 0.1% Triton X-100; 1% DMSO in PBS) for 2 h. Fixed cells are immunostained with antibodies against anti-BrdU mouse monoclonal antibody IgG followed by incubation with the secondary antibodies FITC conjugated goat anti-mouse IgG. The treated cells are covered with slow-fade anti-fade with DAPI for nuclear staining and covered with a glass coverslip. Images are captured with the fluorescence microscope[2]. References [1]. Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8129-34. [2]. Park S, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Anim Reprod Sci. 2015 Oct;161:47-57. Chemical & Physical Properties Density 1.4±0.1 g/cm3 Molecular Formula C15H13N5OS Molecular Weight 311.362 Exact Mass 311.084076 PSA 108.04000 LogP 2.00 Appearance of Characters , white to beige to brown Index of Refraction 1.691 Storage condition Store at -20°C Water Solubility DMSO: soluble20mg/mL, clear |
| Use of | Thiazovivin is a potent ROCK inhibitor, which can protect human embryonic stem cells. Properties Name N-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide Synonym More Synonyms Thiazovivin Biological Activity Description Thiazovivin is a potent ROCK inhibitor, which can protect human embryonic stem cells. Related Catalog Signaling Pathways >> Cell Cycle/DNA Damage >> ROCK Signaling Pathways >> Stem Cell/Wnt >> ROCK Signaling Pathways >> TGF-beta/Smad >> ROCK Research Areas >> Cancer In Vitro Thiazovivin is a ROCK inhibitor. Thiazovivin (2 μM) inhibits ROCK activity and protects human embryonic stem cells (hESCs). Thiazovivin significantly increases the survival of hESCs after dissociation while maintaining pluripotency. Thiazovivin enhances cell-ECM adhesion-mediated integrin signaling. Thiazovivin also stabilizes E-cadherin after cell dissociation to protect hESCs from death under ECM-free conditions[1]. Thiazovivin increases cellular attachment of embryo-derived stem-like cells (eSLCs) of cattle and formation of primary colonies on the feeder layer. Thiazovivin reinforces putative colony outgrowth and supports the expansion of eSLC cultures during the subculture for passaging. Furthermore, Thiazovivin causes greater expression of ectodermal lineage-specific genes in eSLCs of cattle[2]. References [1]. Xu Y, et al. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci U S A. 2010 May 4;107(18):8129-34. [2]. Park S, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Anim Reprod Sci. 2015 Oct;161:47-57. Chemical & Physical Properties Molecular Formula C15H13N5OS Exact Mass 311.084076 PSA 108.04000 Appearance of Characters , white to beige to brown Index of Refraction 1.691 |
| Tax Rebate | 9.0% |
| Supervision | None. MFN tariff: 6.5%. Ordinary tariff: 20.0% |
| Transport Info | Shipping Name: UN |
| MSDS Transport Info | Module 14. Shipping information 14.1 UN dangerous goods number European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.2 United Nations shipping name European Land Transport Dangerous Regulations: Non-dangerous goods IMDG Code: Non-dangerous goods International air transport dangerous goods regulations: non-dangerous goods 14.3 Transport hazard categories European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.4 Package group European Land Transport Dangerous Regulations: -International Maritime Dangerous Regulations: -International Air Transport Dangerous Regulations: - 14.5 Environmental hazards European Land Transport Danger Regulations: No International Maritime Danger Regulations International Air Transport Danger Regulations: No Marine pollutants (yes/no): No 14.6 Special reminder to users No data available |
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